非小细胞肺癌患者外周血中WNT7A甲基化检测的诊断价值

目的 探索WNT7A甲基化和NSCLC临床病理因素的关系。方法 选取120例NSCLC患者为NSCLC组，另外选择120位健康者为对照组，采用甲基化特异性聚合酶链反应（MSP）法检测两组的WNT7A启动子甲基化率，并分析WNT7A甲基化和NSCLC临床病理因素的关系。结果 NSCLC组患者的外周血中WNT7A甲基化率显著高于对照组（P<0.05），WNT7A基因甲基化率与患者的性别、病理类型、年龄、吸烟史等无显著相关性（P>0.05），而与肿瘤大小、远处转移、TNM分期、胸腔积液显著相关（P<0.05）。有胸腔积液、肿瘤直径＞5 cm的NSCLC患者外周血中WNT7A基因甲基化检出率显著高于无胸腔积液、肿瘤直径≤5 cm患者（均P<0.05）；TNM分期为Ⅲ~Ⅳ的NSCLC患者外周血中WNT7A基因甲基化检出率显著高于分期为Ⅰ~Ⅱ的NSCLC患者（P<0.05）。结论 WNT7A基因甲基化在NSCLC患者中检出率较高，可作为NSCLC有效的诊断依据。

Detection of WNT7A Methylation in Peripheral Blood of NSCLC Patients and Its Diagnostic Value

Objective To explore the relation between WNT7A methylation and clinical factors of NSCLC patients. Methods We selected 120 NSCLC patients as NSCLC group and 120 healthy people as control group. The detection rate of WNT7A methylation in two groups was compared by methylation specific polymerase chain reaction, and the relation between WNT7A methylation and clinical factors of NSCLC patients was compared. Results The methylation rate of WNT7A in peripheral blood of NSCLC group was significantly higher than that in control group (P<0.05). In peripheral blood of NSCLC group, there was no significant correlation between WNT7A gene methylation and gender, pathological type, age, smoking history(P>0.05). The methylation rate of WNT7A gene was significantly correlated with tumor size, distant metastasis, TNM stage and pleural effusion(P<0.05). The detection rate of WNT7A gene methylation in peripheral blood of NSCLC patients with pleural effusion, tumor size >5cm and TNM stage Ⅲ-Ⅳ was significantly higher than that of patients without pleural effusion, tumor size ≤5cm and stage Ⅰ-Ⅱ (P<0.05). Conclusion The detection rate of WNT7A gene methylation is high in NSCLC patients, which can be used as an effective diagnostic basis for NSCLC.