泛素结合酶UBE2T在结直肠癌中的表达及其生物学作用

目的探索泛素结合酶UBE2T在结直肠癌的表达及其生物学作用。方法运用免疫组化法检测78对结直肠癌和对应癌旁正常组织中UBE2T的蛋白表达水平。脂质体转染法将UBE2T-siRNA及对照NC-siRNA转染结直肠癌细胞SW480后,采用细胞计数试剂盒(CCK-8)及平板克隆实验分析增殖情况;流式细胞仪检测细胞周期情况;Western blotting检测细胞中UBE2T、cyclin D1、cyclin E等蛋白表达情况。结果与正常结直肠组织相比,结直肠癌组织中UBE2T表达水平增高(P<0.001)。癌组织中UBE2T异常高表达与不良病理指标相关:肿瘤大小(P=0.014)、组织分化程度(P=0.005)、浸润深度(P=0.033)和TNM分期(P=0.014)。CCK-8增殖实验和平板克隆形成实验都提示沉默UBE2T表达能显著抑制结直肠癌细胞的增殖。沉默UBE2T抑制周期蛋白cyclin D1和cyclin E的表达,促进结直肠癌细胞发生G1/S期细胞周期阻滞。结论 UBE2T在结直肠癌组织中异常高表达,并与不良病理指标相关。沉默UBE2T通过促进G1/S期细胞周期阻滞抑制结直肠癌细胞的增殖。

The expression pattern and biological role of ubiquitin-binding enzyme UBE2T in colorectal cancer

Objective To investigate the expression pattern and biological role of ubiquitin-binding enzyme UBE2T in colorectal cancer. Methods Immunohistochemistry was used to detect the protein expression of UBE2T in 78 pair specimens of colorectal cancer and corresponding normal tissues. After UBE2T-siRNA and control NC-siRNA were transfected into colorectal cancer cell SW480 by lipofectamine 2000, proliferation was evaluated by cell counting kit (CCK-8) and plate clone assay. Cell cycle was detected by flow cytometry. The protein expression of UBE2T, cyclin D1 and cyclin E were detected by Western blotting. Results Compared with normal colorectal tissue, the expression of UBE2T in colorectal cancer was significantly increased (P<0.001). The abnormally high expression of UBE2T in cancerous tissues was associated with poor pathological features: tumor size (P=0.014), histological grade (P=0.005), depth of invasion (P=0.033) and TNM stage (P=0.014). Both CCK-8 proliferation assay and plate clone formation assay suggested that silencing UBE2T expression could significantly inhibit the proliferation of colorectal cancer cells. Silencing UBE2T inhibits the expression of cyclin D1 and cyclin E, and promotes G₁/S cell cycle arrest in colorectal cancer cells. Conclusion UBE2T is highly expressed in colorectal cancer tissues and is associated with poor pathological parameters. Silencing UBE2T inhibits the proliferation of colorectal cancer cells by promoting G₁/S cell cycle arrest.