| miR-21通过靶向SOX2增强胃癌细胞的迁移能力 |
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| 引用本文： | 袁 挺1,' target='_blank'>2,韩 川1,' target='_blank'>3,孙妮娜4,刘彩芳4,石 淼4,时永全1. miR-21通过靶向SOX2增强胃癌细胞的迁移能力[J]. 现代肿瘤医学, 2019, 0(19): 3361-3364. DOI: 10.3969/j.issn.1672-4992.2019.19.002 |
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| 作者姓名： | 袁 挺1 ' target='_blank'>2 韩 川1 ' target='_blank'>3 孙妮娜4 刘彩芳4 石 淼4 时永全1 |
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| 作者单位： | 1.空军军医大学西京消化病医院肿瘤生物学国家重点实验室，陕西 西安 710032;2.中国人民解放军第150医院门诊部，河南 洛阳 471000;3.火箭军峨眉疗养院，四川 峨眉 614200;4.西安医学院，陕西 西安 710021 |
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| 基金项目： | National Natural Science Foundation of China（No.81873554,81470805）;国家自然科学基金(编号:81873554,81470805);陕西省创新能力支撑计划(编号:2018TD-003) |
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| 摘 要： | 目的:探讨miR-21在胃癌细胞系中对SOX2的调控作用及其对胃癌细胞迁移的影响。方法:在胃癌细胞系AZ-521、AGS中通过转染miR-21 mimic或者miR-21 antagomir构建miR-21过表达和低表达模型，RT-qPCR检测转染效果，并通过蛋白免疫印迹法检测SOX2在转染细胞中表达的变化。构建miR-21双荧光素酶报告基因，在工具细胞HEK293T中检验miR-21是否通过直接结合SOX2的3′-UTR区域来对其实现调控。应用Transwell迁移实验检测miR-21及SOX2对胃癌细胞迁移能力的影响。结果:miR-21直接结合于SOX2的3′-UTR区域来抑制SOX2在胃癌细胞系中的表达。迁移实验发现上调miR-21或者下调SOX2都能促进胃癌细胞的迁移。结论:miR-21可促进胃癌细胞的迁移能力，其机制可能通过抑制SOX2的表达而实现。
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| 关 键 词： | miR-21 SOX2 胃癌 迁移 |
miR-21 regulates migration of gastric cancer cells via targeting SOX2 |
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| Yuan Ting1,' target='_blank'>2,Han Chuan1,' target='_blank'>3,Sun Nina4,Liu Caifang4,Shi Miao4,Shi Yongquan1. miR-21 regulates migration of gastric cancer cells via targeting SOX2[J]. Journal of Modern Oncology, 2019, 0(19): 3361-3364. DOI: 10.3969/j.issn.1672-4992.2019.19.002 |
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| Authors: | Yuan Ting1 ' target='_blank'>2 Han Chuan1 ' target='_blank'>3 Sun Nina4 Liu Caifang4 Shi Miao4 Shi Yongquan1 |
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| Affiliation: | 1.State Key Laboratory of Cancer Biology，National Clinical ResearchCenter for Digestive Diseases and Xijing Hospital of Digestive Diseases，Airforce Military Medical University，Shaanxi Xi'an 710032，China;2.The 150th Hospital of the People's Liberation Army，Henan Luoyang 471000，China;3.Rocket Army Emei Sanatorium，Sichuan Emei 614200，China;4.College of Postgraduates，Xi'an Medical University，Shaanxi Xi'an 710021，China. |
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| Abstract: | Objective:To investigate the regulation of miR-21 on SOX2 in gastric cancer cell lines and its effect on the migration of gastric cancer cells.Methods:AZ-521 and AGS were transfected with mimic or antagomir to increase or decrease miR-21 expression.The expression of miR-21 was detected by RT-qPCR and the expression of SOX2 was detected by Western blot.To test whether miR-21 can regulate SOX2 by directly binding to its 3'-UTR region.A double luciferase reporter gene of miR-21 was constructed in HEK293T.Transwell assay was used to detect the effects of miR-21 and SOX2 on gastric cancer cell migration.Results:The expression of SOX2 in gastric cancer cell lines was inhibited by the direct binding of miR-21 to the 3'-UTR region of SOX2.We also demonstrated that either up-regulation of miR-21 or down-regulation of SOX2 could promote the migration of gastric cancer cells.Conclusion:miR-21 directly regulates the expression of SOX2 and affects the migration of gastric cancer AGS and AZ-521 cells. |
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| Keywords: | miR-21 SOX2 gastric cancer migration |
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