GSTM1和CYP1A1基因多态性与宫颈癌发生及发展的相关性

目的:探讨谷胱苷肽硫转移酶M1（GSTM1）和CYP1A1 Exon7基因多态性与宫颈癌发生发展的关系。方法:选取2013年5月至2015年5月我院收治的宫颈癌患者184例为宫颈癌组，203例进行体检的健康人群为参照组。用限制性片段长度多态性-聚合酶链反应（RFLP-PCR）检测所有受试者GSTM1和CYP1A1 Exon7基因型；记录无进展生存期（PFS），并随访观察生存和死亡情况。结果:GSTM1分为野生型（wt）和突变缺失型（null），CYP1A1 Exon7野生型为Ⅱe/Ⅱe，突变型包括突变纯合型（Val/Val）、突变杂合型（Ⅱe/Val）。宫颈癌组携带GSTM1突变型基因型比例与参照组间比较，差异无统计学意义（P＞0.05）；宫颈癌组携带CYP1A1 Exon7突变型基因型比例显著高于参照组（P＜0.05），且携带突变型基因型个体患宫颈癌的风险是携带野生型基因型个体的2.333倍。GSTM1、CYP1A1 Exon7基因型与宫颈癌患者年龄、病理分期、肿瘤分化程度、肿瘤直径及淋巴结转移均无关（P＞0.05），与患者病理类型有关（P＜0.05）。GSTM1、CYP1A1 Exon7突变型宫颈癌患者PFS中位数与野生型患者比较，差异均无统计学意义（P＞0.05）。GSTM1、CYP1A1 Exon7突变型宫颈癌患者基因型与宫颈癌患者预后无关（P＞0.05）。结论:GSTM1及CYP1A1 Exon7基因多态性是宫颈癌发生发展的危险因素，尤其是CYP1A1 Exon7突变型，为预防宫颈癌提供依据。

Correlation of GSTM1 and CYP1A1 gene polymorphisms with occurrence and development of cervical cancer

Objective:To investigate the relationship of polymorphisms of glutathione sulfotransferase M1 (GSTM1) and CYP1A1 Exon7 gene with the occurrence and development of cervical cancer.Methods:184 patients with cervical cancer admitted to our hospital from May 2013 to May 2015 were selected as the cervical cancer group and 203 healthy people as the control group.The genotypes of GSTM1 and CYP1A1 Exon7 were detected by restriction fragment length polymorphism-polymerase chain reaction (RFLP-PCR).Progression-free survival (PFS) was recorded,and survival and mortality were followed up.Results:GSTM1 was divided into wild type (wt) and mutant deletion type (null),and CYP1A1 Exon7 wild type was IIe/IIe,and mutant types included mutant homozygous (Val/Val) and mutant heterozygous (IIe/Val).There was no significant difference in the proportion of GSTM1 mutant genotype between the cervical cancer group and the control group (P＞0.05).The proportion of CYP1A1 Exon7 mutant genotype in the cervical cancer group was significantly higher than that in the control group (P＜0.05),and the risk of cervical cancer in individuals carrying mutant genotype was 2.333 times higher in the individuals with wild genotype.GSTM1 and CYP1A1 Exon7 genotypes were not associated with age,pathological stage,tumor differentiation,tumor diameter and lymph node metastasis (P＞0.05),but with pathological type (P＜0.05).There was no significant difference in PFS median between cervical cancer patients with GSTM1 and CYP1A1 Exon7 mutations and those with wild type (P＞0.05).The genotype of GSTM1,CYP1A1 Exon7 mutant cervical cancer patients was not related to the prognosis (P＞0.05).Conclusion:GSTM1 and CYP1A1 Exon7 gene polymorphisms are risk factors for the occurrence and development of cervical cancer,especially CYP1A1 Exon7 mutation,which can provide evidence for prevention of cervical cancer.