错配修复基因MSH3在人晶状体中的表达

目的研究错配修复基因MSH3在人晶状体上皮细胞系SRA01/04、年龄相关性白内障(age-related cataract,ARC)患者晶状体组织以及24周人胎眼晶状体组织中的表达情况,探讨其与ARC形成的关系。方法采用RT-PCR检测人晶状体上皮细胞系SRA01/04、ARC患者晶状体组织和健康人脐带(阳性对照)中MSH3基因的表达水平,免疫荧光检测24周人胎眼晶状体中MSH3蛋白的表达。结果 MSH3在晶状体上皮细胞系SRA01/04中高表达,在ARC患者晶状体组织中表达下调。MSH3在24周人胎眼晶状体纤维细胞、晶状体上皮细胞以及晶状体前囊组织中均有表达,且在晶状体上皮细胞中高表达。结论 MSH3在人胎眼晶状体组织和SRA01/04中高表达,而在ARC患者晶状体组织中表达下调,这种表达差异可能会影响DNA的错配修复过程,进而影响晶状体发育导致ARC的发生。

Expression of DNA mismatch repair gene MSH3 in human lens

Objective　To study the expression of DNA mismatch repair gene MSH3 in lens epithelial cells SRA01/04,lens tissue of age-related cataract (ARC) patients and lens tissue of 24-weeks old human fetal eye,and to explore the relationship between the expression of MSH3 and cataract formation.Methods　RT-PCR was performed to analyze the mRNA expression of MSH3 in SRA01/04 cells，lens tissue of ARC patients and healthy umbilical cord(positive control),and immunofluorescence assay was performed to analyze the expression of MSH3 in 24-weeks old human fetal eye lens.Results　MSH3 was highly expressed in lens epithelial cells SRA01/04 and the expression was decreased in ARC patient’s lens.MSH3 was expressed in 24-weeks old human fetal lens fibroblasts,lens epithelial cells and anterior lens capsule,and especially it was highly expressed in lens epithelial cells.Conclusion　MSH3 is highly expressed in human fetal lens and SRA01/04 cells,but the expression is decreased in lens of ARC patients.The expression difference may affect the process of DNA mismatch repair and development of lens,which can lead to ARC.