长链非编码RNA DLX6-AS1在结肠癌组织与细胞中的表达及其临床意义

目的:检测长链非编码RNA DLX6-AS1(distal-less homeobox 6 antisense 1)在结肠癌组织和细胞中的表达,探讨DLX6-AS1与结肠癌临床病理特征及预后间的关系。方法:利用RT-PCR检测结肠癌组织、细胞及配对癌旁组织、黏膜上皮细胞中DLX6-AS1的表达;利用单因素方差分析DLX6-AS1与结肠癌患者临床病理特征之间的关系;利用生存曲线分析DLX6-AS1与结肠癌患者术后5年生存率的关系;利用Cox风险比例模型分析DLX6-AS1与结肠癌患者预后的关系。结果:DLX6-AS1在结肠癌组织、细胞中表达水平高于癌旁组织和结肠上皮细胞;DLX6-AS1的表达与结肠癌TNM分期相关(P<0.05);DLX6-AS1高表达的结肠癌患者术后5年生存率低于低表达的患者,DLX6-AS1表达是结肠癌患者独立的预后因素。结论:DLX6-AS1在结肠癌中表达升高,并与结肠癌患者生存及预后密切相关。

Expression and clinical significance of long non-coding RNA DLX6-AS1 in colon cancer tissues and cells

Objective:To investigate the expression level of long non-coding RNA（lncRNA) DLX6-AS1 in colon cancer，and evaluate its prognostic value in colon cancer.Methods:Colon cancer cells and colon mucosal cells were tested in this study.Cancer tissue as well as adjacent normal tissue from patients diagnosed with colon cancer were also harvested in this study.Expression of DLX6-AS1 was measured by real-time PCR.ANOVA analysis was performed to evaluate the association between DLX6-AS1 and clinical manifestation of colon cancer patients.K-M survival analysis was utilized to determine correlation of DLX6-AS1 expression with 5-year survival.Cox's proportional hazards regression models were applied to analyze the relationships between DLX6-AS1 and prognosis of colon cancer patients.Results:DLX6-AS1 was upregulated in colon cancer tissues and cells compared with normal tissues and cells.Expression of DLX6-AS1 was closely associated with TNM stage of colon cancer（P<0.05).Expression of DLX6-AS1 was negatively correlated with 5-year survival rate.DLX6-AS1 is an independent prognostic risk factor of colon cancer.Conclusion:LncRNA DLX6-AS1 is upregulated in colon cancer and is negatively associated with survival outcome of colon cancer.