异丙酚调控小儿急性髓系白血病细胞增殖、凋亡的机制研究

目的:研究异丙酚对急性早幼粒细胞白血病细胞HL60增殖、凋亡的影响,并探讨其机制。方法:不同浓度的异丙酚(0、1.5、2.2、3.2μl/ml)处理的HL60细胞,分被标记为C组、P1、P2、P3组;运用MTT法检测各组细胞的细胞抑制率;流式细胞术检测各组细胞的凋亡率;Western blot检测各组细胞中Cleaved PARP、cytochrome c的蛋白表达;比色法检测各组细胞caspase3、caspase9活性。结果:与C组相比,P1、P2、P3组细胞抑制率、细胞凋亡率、caspase3和caspase9活性及CleavedPARP、cytochromec的蛋白表达均显著升高(P<0.05),与P1组相比,P2、P3组细胞抑制率、细胞凋亡率、caspase3和caspase9活性及CleavedPARP、cytochrome c的蛋白表达均显著升高(P<0.05),与P2组相比,P3组细胞抑制率、细胞凋亡率、caspase3和caspase9活性及Cleaved PARP、cytochrome c的蛋白表达均显著升高(P<0.05)。结论:异丙酚可抑制HL60细胞增殖并促进凋亡,其机制可能与上调CleavedPARP、cytochrome c,激活caspase3和caspase9有关,将为异丙酚作为新药治疗急性早幼粒细胞白血病的开发提供依据。

The mechanism of propofol regulating proliferation and apoptosis of acute myeloid leukemia cells in children

Objective:To study the effect of propofol on cell proliferation and apoptosis of acute promyelocytic leukemia cell line HL60，and explore its mechanism.Methods:HL60 cells treated with different concentrations of propofol (0，1.5，2.2，3.2 μl/ml) were labeled as group C，group P1，group P2 and group P3.Cell inhibition of each group was detected by MTT assay.The apoptosis rate of each group was detected by flow cytometry.The protein expression of Cleaved PARP and cytochrome c in each group was detected by Western blot.The activity of caspase 3 and caspase 9 in each group was detected by colorimetry.Results:Compared with group C，the cell inhibition rate，apoptosis rate，caspase 3 and caspase 9 activity，and Cleaved PARP and cytochrome c protein expression in P1，P2 and P3 group were significantly increased (P<0.05).Compared with the P1 group，the cell inhibition rate，apoptosis rate，caspase 3 and caspase 9 activities，and Cleaved PARP and cytochrome c protein expression in the P2 group and the P3 group were significantly higher (P<0.05).Compared with group P2，the cell inhibition rate，apoptosis rate，caspase 3 and caspase 9 activity，and the protein expression of Cleaved PARP and cytochrome c in P3 group were significantly increased (P<0.05).Conclusion:Propofol can inhibit proliferation and promote apoptosis of HL60 cells，which may be related to up-regulation of Cleaved PARP，cytochrome c，activation of caspase 3 and caspase 9，and will provide a basis for the development of acute granulocytic leukemia with propofol as a new drug.