子宫内膜癌中FasL和Ki67的表达及临床意义

目的:检测子宫内膜癌组织中脂肪酸合成酶配体(fatty acid synthase ligand,FasL)和细胞增殖核抗原(Ki67)的表达情况,探讨二者表达的相关性及临床意义。方法:搜集2012年6月至2015年8月在我院确诊并治疗的82例子宫内膜癌标本作为研究对象,同期选取24例非典型增生子宫内膜标本和30例正常子宫内膜标本作为对照。采用免疫组化法检测不同子宫内膜组织中Ki67、FasL蛋白表达水平,分析Ki67、FasL与子宫内膜癌各项临床病理参数的相关性。采用Spearman对Ki67与FasL的相关性进行分析。结果:Ki67、FasL在正常子宫内膜、非典型增生内膜及子宫内膜癌组织中阳性率均逐渐增加,两两比较差异有统计学意义(P<0.05)。Ki67、FasL表达与子宫内膜癌患者FIGO分期、病理分级、淋巴结转移及肌层浸润密切相关(P<0.05),与患者年龄、月经状态、脉管瘤栓形成无明显相关性(P>0.05)。经Spearman相关性分析,子宫内膜癌组织中Ki67与FasL表达呈正相关性(r=0.438,P<0.05)。Ki67高表达者2年存活率明显低于低表达者(61.76%vs 92.86%,P<0.05);FasL高表达者2年存活率明显低于低表达者(57.14%vs 88.46%,P<0.05)。结论:Ki67、FasL在子宫内膜癌组织中显著高表达,有望共同作为诊断子宫内膜癌及判断患者预后的有效生物标志物。

Expression of FasL and Ki67 in endometrial carcinoma and its clinical significance

Objective:To explore proliferating cell nuclear antigen (Ki67)and fatty acid synthase ligand (FasL)expression in endometrial carcinoma，and analyze the relationship between the two and clinical pathological parameters.Methods:82 cases of endometrial cancer specimens confirmed and treated in our hospital from June 2012 to August 2015 were selected as the study objects，and 24 cases of atypical endometrial specimens and 30 cases of normal endometrial specimens in the same period were as the control.The levels of Ki67 and FasL proteins expression in different endometrial tissues were detected by using immunohistochemical method，and the correlation of Ki67，FasL and clinicopathological parameters of endometrial carcinoma was analyzed，and the correlation between Ki67 and FasL was analyzed by using Spearman.Results:The positive rates of Ki67 and FasL in normal endometrium，atypical endometrium hyperplasia and endometrial carcinoma were increased gradually，with statistically significant difference (P<0.05).The expression of Ki67 and FasL was closely related to FIGO staging，pathological grading，lymph node metastasis and myometrial invasion of patients with endometrial carcinoma (P<0.05)，and was not significantly related to patients' age，menstrual status and formation of vascular tumor thrombus (P>0.05).Spearman correlation analysis showed the expression of Ki67 was positively correlated with FasL (r=0.438，P<0.05).The 2-year survival rate of patients with high expression of Ki67 was 61.76%，which was lower than that of low expression of Ki67 significantly (92.86%)(P<0.05).The 2-year survival rate of patients with high expression of FasL is much lower than that of low expression of FasL (57.14% vs 88.46%，P<0.05).Conclusion:Ki67 and FasL showed significant high-expression in endometrial carcinoma tissues，and were closely related with FIGO staging，pathological grading，lymph node metastasis and myometrial invasion of endometrial carcinoma.It is hopeful to be used as an effective marker for early diagnosis and treatment of endometrial carcinoma.