线粒体动力相关蛋白Drp1对阿尔茨海默病早期诊断价值的研究

目的探究血浆和脑脊液线粒体动力学相关蛋白(dynamic-related protein 1,Drp1)作为生物标志物在阿尔茨海默病(AD)早期诊断中的临床价值。方法选取2015年6月—2017年6月就诊于杭州市第七人民医院老年科125例患者为研究对象,其中轻度认知功能障碍(mild cognitive impairment,MCI)患者67例,AD患者58例,32例认知功能正常的健康老人为对照组。采用简易智能状态检查量表(MMSE)、蒙特利尔认知评估量表(MoCA)、日常生活能力量表(ADL)进行认知功能评估;Western blotting测定Drp1与SNO-Drp1蛋白。结果 MCI组、AD组、对照组MMSE、MoCA评分差异有统计学意义(均P<0.05)。AD组血浆Drp1显著低于对照组(P<0.05)。脑脊液SNO-Drp1在AD组的表达高于MCI组与对照组(均P<0.05),AD组SNO-Drp1/Drp1值高于对照组。ROC曲线预测MCI的灵敏度和特异度分别是77.6%和56.2%,预测AD的灵敏度和特异度分别是86.2%和87.5%。结论 AD患者脑脊液SNO-Drp1/Drp1比值诊断AD的敏感度和特异度较高且接近,在早期诊断AD方面具有推广价值。

Value of mitochondrial dynamic protein 1 in the early diagnosis of Alzheimer's disease

Objective To explore the clinical value of plasma and cerebrospinal fluid Dynamic-related protein 1(Drp1) as biomarkers in the early diagnosis of Alzheimer's disease. Methods Total 125 patients in the department of geriatrics of Hangzhou 7th people's hospital from June 2015 to June 2017 were selected as subjects, including 67 cases of mild cognitive impairment(MCI), 58 cases of AD, and 32 normal control as control. The cognitive function score were assayed according to Mini-Mental State Examination (MMSE), Montreal Cognitive Assessment (MoCA) and Activity of Daily Living Scale (ADL). The levels of Drp1 and SNO-Drp1 in plasma and cerebrospinal fluid were tested by using Western blot. Results There was significant difference in MMSE and MoCA scores among the three groups (P<0.05). The plasma Drp1 of AD group was significantly lower than that of the control group (P<0.05). The expression of SNO-Drp1 in cerebrospinal fluid in AD group was significantly higher than those in the MCI group and control group (P<0.05), and the SNO-Drp1/Drp1 ratio in AD group was significantly higher than that in the control group. The sensitivity and specificity by ROC curve for MCI were 77.6% and 56.2%, and for AD were 86.2% and 87.5%. Conclusion The sensitivity and specificity of cerebrospinal fluid SNO-Drp1/Drp1 ratio for AD diagnosis is high and proximate, which is worthy of promotion.