上调FoxM1增强骨髓间充质干细胞抗脂多糖诱导肺泡上皮细胞凋亡

【目的】本研究旨在探讨上调骨髓间充质干细胞（BMSC）的FoxM1表达水平对其抗脂多糖诱导肺泡上皮细胞凋亡作用的影响，并初步探索其可能机制。【方法】采用全骨髓细胞贴壁培养法分离获取大鼠BMSC，通过慢病毒转染技术使BMSC过表达FoxM1，利用Transwell小室构建BMSC与肺泡上皮细胞共培养体系，使用TUNEL法与Annexin V法检测不同FoxM1表达水平BMSC的抗脂多糖诱导肺泡上皮细胞凋亡作用，采用MILLIPLEXMAP液相芯片检测共培养体系中多种细胞因子的表达水平。【结果】TUNEL与Annexin V检测结果显示，相较于野生型BMSC，与过表达FoxM1的BMSC共培养的肺泡上皮细胞受脂多糖刺激后凋亡水平更低，差异均有统计学意义（P<0.05）。MILLIPLEXMAP液相芯片检测显示，上调BMSC的FoxM1表达水平可使其与肺泡上皮细胞的共培养体系中IL-13、IL-21、IL-23、MIP-1a、MIP-1b表达水平升高，而使MIP-3a表达水平降低。【结论】上调FoxM1表达水平可增强BMSC抗脂多糖诱导肺泡上皮细胞凋亡作用，可能与其改变肺泡上皮细胞某些炎症因子的释放水平有关。

Over-expression of BMSC FoxM1 Attenuates LPS-induced Apoptosis of Alveolar Epithelial cells

【Objective】 To investigate whether bone marrow mesenchymal stem cells （BMSC） over- expressing FoxM1 gene can attenuate lipopolysaccharide（LPS）-induced apoptosis of alveolar epithelial cells，and explore its possible mechanism. 【Methods】 SD rat BMSC were isolated and cultured by whole bone marrow adherence method. FoxM1 gene was overexpressed in BMSC by lentiviral transfection. The expression of the target gene FoxM1 was verified by Western blot. Apoptosis of A549 cells was measured by TUNEL and flow cytometry. And the multi- factor level of supernatant in BMSC- A549 co- culture system was detected by Milliplex method. 【Results】TUNEL and flow cytometry confirmed that the apoptosis rate of A549 induced by LPS decreased after co-culture with BMSC overexpressing FoxM1，and the difference was statistically significant（P < 0.05）. Milliplex assay showed that the levels of IL- 13，IL-21，IL-23，MIP-1a， MIP- 1b and in BMSC overexpressing FoxM1 gene and A549 co- culture system were significantly increased，while the MIP-3a level is significantly reduced.【Conclusion】BMSC overexpressing FoxM1 gene can attenuate LPS-induced apoptosis of alveolar epithelial cells. BMSC may play an anti- apoptotic role by changing the levels of inflammation- related cytokines released by A549 cells.