纳米珍珠粉对小鼠骨髓间充质干细胞活性影响研究

目的探讨纳米珍珠粉(nano-pearl powder,NPP)对小鼠骨髓间充质干细胞活性的影响。方法研究于2016年8月至2017年3月在中南大学湘雅医学院附属海口医院实验中心进行。将NPP以100、300、500μg/mL的质量浓度与小鼠骨髓间充质干细胞共培养24 h(NPP组),用CCK8法测定吸光度值,计算细胞相对增殖率,评定其细胞毒性级别;用Annexin-FITC/PI法检测细胞凋亡率。两者综合评定NPP对小鼠骨髓间充质干细胞活性的影响,并与相同质量浓度纳米羟基磷灰石(nano-hydroxyapatite,NHA)(NHA组)进行对比。结果 NPP与NHA细胞毒性检测均合格,NPP在100μg/mL时细胞相对增殖率最高;NPP组细胞凋亡率均低于NHA组,在质量浓度为100和500μg/mL时的差异有统计学意义(P <0.01)。结论 NPP细胞毒性检测合格,其对小鼠骨髓间充质干细胞活性的影响优于NHA。

Effect of nano-pearl powder on cell viability of mouse bone marrow mesenchymal stem cells

Objective    To investigate the effect of nano-pearl powder（NPP） on cell viability of mouse bone marrow mesenchymal stem cells. Methods    Mouse bone marrow mesenchymal stem cells（mBMMSCs）were co-cultured with NPP at concentration of 100，300，500 μg/mL for 24 h （NPP group）. CCK8 test was carried out to evaluate the relative growth rate and cytotoxicity. The apoptosis rate of cells was measured by Annexin-FITC/PI method. The effect of NPP on mBMMSCs activity was analyzed by the above two approaches，and the results were compared with nano-hydroxyapatite（NHA group）at the same concentration. Results    The cytotoxicity tests of NPP and NHA were qualified，and the relative proliferation rates of mBMMSCs in NPP groups were highest at 100 μg/mL. The apoptotic rates of mBMMSCs in NPP groups were all lower than those in NHA groups. The difference was statistically significant at 100 and 500 μg/mL（P < 0.01）. Conclusion    The cytotoxicity tests of NPP are qualified，and its effect on the cell viability of mBMMSCs is better than that of NHA.