| 下调PHLDB3对胃癌耐药细胞系SGC7901/ADR药物敏感性的影响 |
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| 引用本文： | 刘家铭1,张静雯2,司望利1. 下调PHLDB3对胃癌耐药细胞系SGC7901/ADR药物敏感性的影响[J]. 现代肿瘤医学, 2019, 0(17): 3010-3014. DOI: 10.3969/j.issn.1672-4992.2019.17.007 |
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| 作者姓名： | 刘家铭1 张静雯2 司望利1 |
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| 作者单位： | 1.西安市中心医院消化科，陕西 西安 710003;2.西安市第五医院风湿免疫六病区，陕西 西安 710082 |
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| 摘 要： | 目的:探讨下调PHLDB3对胃癌耐药细胞系 SGC7901/ADR 药物敏感性的影响。方法:采用实时定量聚合酶链反应（qRT-PCR）和蛋白免疫印迹技术（Western blot）的方法检测胃癌及癌旁正常组织中PHLDB3的表达（分别是20对和10对）；qRT-PCR和Western blot检测PHLDB3在胃癌细胞系 SGC7901以及胃癌耐药细胞系SGC7901/ADR和SGC7901/VCR中的表达；MTT法检测SGC7901、SGC7901/ADR及转染PHLDB3 siRNA后 SGC7901/ADR的半数抑制浓度（IC50）值；流式细胞仪检测细胞凋亡。结果:qRT-PCR和Western blot结果显示:PHLDB3在胃癌组织中的表达明显高于癌旁正常组织（P<0.000 1）；PHLDB3在耐药细胞系SGC7901/ADR和SGC7901/VCR中的表达明显高于其亲本细胞系SGC7901（P<0.000 1和P<0.05）。Western blot结果显示:相对于转染 PHLDB3 negative control（NC）组，转染 PHLDB3 siRNA组的SGC7901/ADR细胞中PHLDB3的表达降低（P<0.005）。MTT结果显示:SGC7901与 SGC7901/ADR的IC50值分别为（1.5±0.1） μg/ml与（5.5±0.2） μg/ml（P<0.000 1）；SGC7901/ADR 转染 PHLDB3 siRNA 后对顺铂的IC50值明显下降（P<0.05）。流式细胞术（flow cytometry，FCM）检测凋亡，结果显示:下调PHLDB3的表达后，SGC7901/ADR细胞的凋亡率明显增加（P<0.05）。结论:PHLDB3能够促进胃癌细胞系 SGC7901/ADR产生多药耐药。
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| 关 键 词： | PHLDB3 胃癌 多药耐药 |
The effect of PHLDB3 downregulation on drug resistance in gastric cancer cell line SGC7901/ADR |
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| Liu Jiaming1,Zhang Jingwen2,Si Wangli1. The effect of PHLDB3 downregulation on drug resistance in gastric cancer cell line SGC7901/ADR[J]. Journal of Modern Oncology, 2019, 0(17): 3010-3014. DOI: 10.3969/j.issn.1672-4992.2019.17.007 |
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| Authors: | Liu Jiaming1 Zhang Jingwen2 Si Wangli1 |
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| Affiliation: | 1.Digestive Department，Xi'an Central Hospital，Shaanxi Xi'an 710003，China；2.Department of Rheumatology and Immunology，Xi'an No.5 Hospital，Shaanxi Xi'an 710082，China. |
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| Abstract: | Objective:To investigate the influence of PHLDB3 downregulation on drug resistance in gastric cancer drug-resistant cell line SGC7901/ADR．Methods:The expression of PHLDB3 in gastric cancer and adjacent normal tissues was determined by qRT-PCR（20 pairs) and Western blot（10 pairs).The expression of PHLDB3 in gastric cancer cell lines SGC7901，drug-resistant cell line SGC7901/ADR and SGC7901/VCR was detected by qRT-PCR and Western blot.The influence of PHLDB3 knock-down on the IC50 and cell apoptosis of drug-resistant cell line SGC7901/ADR was evaluated using MTT assay and FCM．Results:qRT-PCR and Western blot showed that PHLDB3 was upregulated in gastric cancer compared with adjacent normal tissues（P<0.000 1)，and the expression of PHLDB3 was higher in drug-resistant cell line SGC7901/ADR and SGC7901/VCR than that in SGC7901（P<0.000 1 and P<0.05).Compared to SGC7901/ADR transfected with PHLDB3 NC，SGC7901/ADR transfected with PHLDB3 siRNA showed lower PHLDB3 expression（P<0.005).In addition，MTT assay and FCM showed that the decreased IC50 and increased cell apoptosis rate were observed in drug-resistant cell line SGC7901/ADR with PHLDB3 knock-down(P<0.05).Conclusion:PHLDB3 could contribute to the multidrug resistance of gastric cancer cell line SGC7901/ADR. |
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| Keywords: | PHLDB3 gastric cancer multidrug resistance |
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