维生素C对邻苯二甲酸二丁酯雄性大鼠生殖毒性的保护作用

目的观察邻苯二甲酸二丁酯(di-butylphthalate,DBP)所致雄性生殖毒性效应,并探讨维生素C(vitamin C,Vit C)对DBP所致SD雄性大鼠生殖功能受损的干预调节作用。方法将60只SD雄性大鼠随机分组,分别为正常对照组,DBP低、中、高剂量组(DBP 100、500、1 000 mg/kg体重),VitC对照组(30 mg/kg体重)与VitC与DBP高剂量联合干预组。灌胃干预21 d,检测大鼠脏器重量及血液生化各项指标,大鼠精子活力、浓度、畸形率,以及检测血清睾酮(T)、黄体生成素(LH)、卵泡刺激素(FSH)水平。结果与正常对照组相比,DBP高剂量组大鼠睾丸减轻,睾丸脏体比下降,部分血生化指标异常,精子活力下降、畸形率升高,血清T、LH、FSH水平显著下降,差异有统计学意义(均P<0.05);与DBP高剂量组相比,VitC干预组大鼠睾丸组织损伤有不同程度的恢复,精子活力、畸形率,以及血清T、LH、FSH水平差异有统计学意义(均P<0.01)。结论 DBP可致大鼠睾丸萎缩,精子活力下降,精子畸形率增高;DBP可致血清T、LH、FSH水平降低。VitC可提高血清LDH水平和T、LH、FSH水平,改善DBP所致大鼠精子活力下降,降低精子畸形率,为进一步开展DBP生殖毒性的预防干预提供理论依据。

Protective effect of vitamin C on reproductive toxicity of dibutyl phthalate in male sprague dawley rats

Objective To study the possible pathogenesis of infertility caused by di-butyl phthalate (DBP) and investigate the effects of Vitamin C (Vit C) on DBP-induced reproductive function damage in male Sprague Dawley (SD) rats. Methods Sixty male SD rats were divided into 6 groups, including normal control, Vit C (30 mg/kg BW), DBP (100, 500, 1 000 mg/kg BW), and DBP (1 000 mg/kg BW)+Vit C (30 mg/kg BW) groups with ten rats in each group. After the treatment conducted by DBP and Vit C for three weeks, the rats were sacrificed and testicular tissues were collected to calculate organ coefficient and determine sperm motility, concentration, deformity rate. And the index including hematology, and serum chemistry, serum T, LH, FSH concentration were detected. Results Compared with the normal group, the testes of the high-dose DBP group showed obvious atrophy, LDH concentration significantly decreased, the sperm motility and concentration decreased significantly, and the deformity rate of sperm increased, the concentration of T, LH, FSH in serum decreased significantly. Conclusion DBP can cause testicular atrophy in rats, sperm motility, and sperm deformity rate increases. DBP can reduce serum T and LH levels and increase FSH levels. Vit C can improve DBP-induced productive function injury, increase sperm motility and decrease sperm abnormality, elevate the level of serum T, LH, and FSH, and thus promote the reproductive function of the male rat.