黄芩苷通过Notch通路对人结肠癌SW480细胞增殖和凋亡的影响

目的:探讨黄芩苷能否通过抑制人结肠癌SW480细胞中Notch通路的表达而抑制其增殖并促进其凋亡。方法:MTT法及流式检测术分别检测不同浓度黄芩苷对人结肠癌SW480细胞生长抑制及凋亡的影响;Western Blot法及RT-PCR法分别检测不同浓度黄芩苷对人结肠癌SW480细胞Notch1、Hes-1蛋白和Jagged1基因表达的影响。结果:随着药物剂量的增大,黄芩苷对人结肠癌SW480细胞的抑制率逐步上升;黄芩苷20μmol/L组细胞凋亡率显著高于对照组;黄芩苷40μmol/L组细胞凋亡率显著高于黄芩苷20μmol/L组;黄芩苷20μmol/L组细胞Notch1、Hes-1蛋白、Jagged1基因表达均显著低于对照组,黄芩苷40μmol/L组Notch1、Hes-1蛋白、Jagged1基因表达均显著低于黄芩苷20μmol/L组。结论:黄芩苷能抑制人结肠癌SW480细胞的增殖,促进其凋亡,这种作用可能是通过对细胞中Notch通路的负性调节而实现的。

Effect of baicalin on proliferation and apoptosis of human colorectal cancer SW480 cells via Notch pathway

Objective:To investigate whether baicalin can inhibit the proliferation and promote apoptosis of human colorectal cancer SW480 cells by inhibiting the Notch pathway.Methods:MTT assay and flow cytometry were used to detect the effects of different concentrations of baicalin on the growth inhibition and apoptosis of human colorectal cancer SW480 cells.Western Blot and RT-PCR methods were used to detect different concentrations of baicalin on Notch1,Hes-1 and Jagged1 expression of human colorectal cancer SW480 cells.Results:With the increase of drug dosage,the inhibitory rate of baicalin on human colorectal cancer SW480 cells increased gradually.The apoptosis rate of baicalin 20 μmol/L group was significantly higher than that of the control group.The apoptosis rate of baicalin 40 μmol/L group was significantly higher than that of baicalin 20 μmol/L group.Notch1,Hes-1 protein,Jagged1 gene expression of baicalin 20 μmol/L group were significantly lower than the control group,and expression of Notch1,Hes-1 protein,Jagged1 gene of baicalin 40 μmol/L group were significantly lower than that of baicalin 20 μmol/L group.Conclusion:Baicalin can inhibit the proliferation of human colorectal cancer SW480 cells and promote its apoptosis.This effect may be achieved through the negative regulation of Notch pathway in cells.