miR-367-3p在非小细胞肺癌组织中的表达及对细胞功能的影响

目的:研究miR-367-3p在非小细胞肺癌（non-small cell lung cancer，NSCLC）中促进肿瘤生长的作用机制。方法:收集非小细胞肺癌患者的临床标本，检测miR-367-3p在非小细胞肺癌组织中的表达。利用qRT-PCR、MTT和流式细胞术分别评估miR-367-3p的表达改变对肿瘤细胞增殖和周期的影响。Western blot检测miR-367-3p表达改变对FBXW7的影响。双荧光素酶实验验证miR-367-3p与FBXW7之间的直接关系。结果:miR-367-3p在非小细胞肺癌组织中的表达明显高于对应癌旁组织。miR-367-3p作为促癌的miRNA，在非小细胞肺癌细胞中促进肿瘤细胞增殖和细胞周期进程。miR-367-3p对NSCLC细胞生物学功能的影响部分依赖靶向抑制FBXW7。结论:miR-367-3p通过靶向抑制FBXW7的表达促进NSCLC细胞增殖和周期。

Expression of miR-367-3p in non-small cell lung cancer and its effect on cell function

Objective:To study the mechanism of miR-367-3p promoting the tumor growth in non-small cell lung cancer (NSCLC).Methods:The clinical specimens of patients with NSCLC were collected to detect the expression of miR-367-3p in NSCLC tissues.qRT-PCR，MTT and flow cytometry were used to evaluate the effects of miR-367-3p on cell proliferation and cell cycle in NSCLC cells.The effect of miR-367-3p on FBXW7 was detected by Western blot.Using dual luciferase experiment to verify the direct relationship between miR-367-3p and FBXW7.Results:The expression of miR-367-3p in NSCLC tissues was significantly higher than that in corresponding paracancerous tissues.As an onco-miRNA，miR-367-3p promoted cell proliferation and cell cycle progression.Effect of miR-367-3p on biological function of NSCLC cells partially depended on targeting inhibition of FBXW7.Conclusion:miR-367-3p plays a tumorigenic role in NSCLC by targeting FBXW7.