| 弓形虫致鼠胚神经系统畸形中NCAM的表达及青蒿素保护作用的研究 |
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| 引用本文： | 孙秀宁,管英俊,张皓云,史立宏,汲蕊,李瑞芳,管志玉. 弓形虫致鼠胚神经系统畸形中NCAM的表达及青蒿素保护作用的研究[J]. 中国病原生物学杂志, 2011, 0(7) |
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| 作者姓名： | 孙秀宁 管英俊 张皓云 史立宏 汲蕊 李瑞芳 管志玉 |
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| 作者单位： | 潍坊医学院病原生物学教研室;潍坊医学院组织胚胎学教研室;潍坊医学院人体解剖学教研室;潍坊医学院药理学教研室; |
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| 基金项目： | 潍坊市科技发展计划项目(No.200902062) |
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| 摘 要： | 目的探讨弓形虫感染孕早期大鼠致胚胎神经系统畸形的分子机制及青蒿素对胚胎的保护作用。方法将SD孕鼠随机分为对照组、模型组和模型治疗组,各组孕鼠于妊娠第3 d(E3)腹腔注射弓形虫RH强毒株,并分别于E12、E14、E16、E18剖腹取胎鼠,进行形态学观察;PCR法检测羊水中弓形虫B1基因表达,免疫荧光染色观察神经细胞粘附分子(NCAM)在胚胎不同发育时期的神经细胞中的表达规律。结果模型组胚胎发育状况差,活胎率低,为11.82%;PCR检测到模型组胚胎羊水B1基因的表达。对照组和模型治疗组E14、E16鼠胚神经细胞中NCAM呈阳性,模型组染色较其他4组弱。结论 NCAM低表达是神经系统畸形发生的重要因素,青蒿素对鼠胚弓形虫感染有保护作用。
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| 关 键 词： | 弓形虫 神经系统畸形 青蒿素 神经细胞粘附分子 |
A study on the molecular mechanism of nervous system defects induced by Toxoplasma gondii during embryonic development and the roles of artemisinin |
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| SUN Xiu-ning,GUAN Ying-jun,ZHANG Hao-yun,SHI Li-hong,JI Rui,LI Rui-fang,GUAN Zhi-yu. A study on the molecular mechanism of nervous system defects induced by Toxoplasma gondii during embryonic development and the roles of artemisinin[J]. Journal of Pathogen Biology, 2011, 0(7) |
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| Authors: | SUN Xiu-ning GUAN Ying-jun ZHANG Hao-yun SHI Li-hong JI Rui LI Rui-fang GUAN Zhi-yu |
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| Affiliation: | SUN Xiu-ning1,GUAN Ying-jun2,ZHANG Hao-yun3,SHI Li-hong4,JI Rui1,LI Rui-fang1,GUAN Zhi-yu1 (1.Department of Pathogen Biology,Weifang Medical College,Weifang 261053,Shandong,China,2.Department of Histology and Embryology,3.Department of Anatomy,4.Department of Pharmacology,Weifang Medical College) |
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| Abstract: | Objective To explore the molecular mechanism of nervous system defects induced by Toxoplasma gondii and to observe the roles of artemisinin.Methods Pregnant SD rats were divided randomly into a normal control group,a model group,and a treatment group.SD rats were infected with tachyzoites of T.gondii by intra-peritoneal injection on day 3 of pregnancy(E3) and were sacrificed on E12,E14,E16,and E18.The morphological changes in fetuses were observed.T.gondii DNA in amniotic fluid was detected with PCR.The exp... |
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| Keywords: | Toxoplasma gondii neural system defect artemisinin neural cell adhesion molecule(NCAM) |
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