| H9亚型禽流感病毒HA基因的克隆与序列分析 |
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| 引用本文： | 李林,桑晓宇,朱晓文,于志君,李天松,王磊,黄海楠,阮洋,秦峻岭,刘玉秀,高玉伟,夏咸柱,胡桂学. H9亚型禽流感病毒HA基因的克隆与序列分析[J]. 中国病原生物学杂志, 2011, 0(5) |
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| 作者姓名： | 李林 桑晓宇 朱晓文 于志君 李天松 王磊 黄海楠 阮洋 秦峻岭 刘玉秀 高玉伟 夏咸柱 胡桂学 |
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| 作者单位： | 吉林农业大学;军事医学科学院军事兽医研究所吉林省人兽共患病预防与控制重点实验室; |
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| 基金项目： | 国家“十一五”重大传染病专项(No.2009zx10004-4001) |
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| 摘 要： | 目的扩增、克隆H9N2 HA基因,并进行序列分析,为H9N2流感病毒种间及跨种传播机制研究奠定基础。方法设计特异性引物,扩增克隆H9N2亚型禽流感病毒,测序后进行序列分析。结果成功克隆出4株H9N2亚型禽流感病毒,全长均为1.7 kb,ORF为1 683 bp,编码560个氨基酸;联机检索,与参考毒株比对核苷酸和氨基酸同源性均在90%以上。结论成功克隆4株H9N2亚型禽流感病毒,序列分析显示其核苷酸和推导氨基酸与参考毒株高度同源。
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| 关 键 词： | H9亚型禽流感 HA基因 克隆 序列分析 |
Cloning and sequencing analysis of HA genes with H9 subtype influenza virus |
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| LI Lin,,SANG Xiao-yu,ZHU Xiao-wen,YU Zhi-jun,LI Tian-song,WANG Lei,HUANG Hai-nan,RUAN Yang,QIN Jun-ling,LIU Yu-xiu,GAO Yu-wei,XIA Xian-zhu,HU Gui-xue. Cloning and sequencing analysis of HA genes with H9 subtype influenza virus[J]. Journal of Pathogen Biology, 2011, 0(5) |
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| Authors: | LI Lin SANG Xiao-yu ZHU Xiao-wen YU Zhi-jun LI Tian-song WANG Lei HUANG Hai-nan RUAN Yang QIN Jun-ling LIU Yu-xiu GAO Yu-wei XIA Xian-zhu HU Gui-xue |
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| Affiliation: | LI Lin1,2,SANG Xiao-yu2,ZHU Xiao-wen1,YU Zhi-jun2,LI Tian-song2,WANG Lei1,HUANG Hai-nan2,RUAN Yang1,QIN Jun-ling2,LIU Yu-xiu2,GAO Yu-wei2,XIA Xian-zhu2,HU Gui-xue2(1.Jilin Agriculture University,Changchun 130000,China,2 Military Veterinary Institute,AMMS,Key Laboratory of Jilin Province for Zoonosis Prevention and Control,Changchun 130122,China) |
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| Abstract: | Objective Amplified,cloned,and analyzed the full-length sequence of the HA gene of four strains of H9N2.Methods A pair of specific primers was designed and the HA gene of four strains H9N2 AIV was cloned and its sequence analyzed.Results The full-length HA of the four strains of H9N2 AIV are all 1.7 kb,with an ORF of 1 683 bp and encoding 560 amino acids.Nucleotide and amino acid homology were both above 90%.Conclusion The HA gene of four strains of H9N2 AIV was successfully cloned and its sequence analyzed... |
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| Keywords: | H9 subtype influenza HA gene clone sequence analysis |
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