| 人乳头瘤病毒快速检测分型方法的临床应用 |
| |
| 引用本文： | 凌云,冯春颜,胡纪文,孙兴宝,罗裕旋,伍月红,幸碧芬,朱彩云. 人乳头瘤病毒快速检测分型方法的临床应用[J]. 中国病原生物学杂志, 2011, 0(7) |
| |
| 作者姓名： | 凌云 冯春颜 胡纪文 孙兴宝 罗裕旋 伍月红 幸碧芬 朱彩云 |
| |
| 作者单位： | 深圳市龙华人民医院妇产科;深圳市龙华人民医院检验科;深圳市菲鹏生物研究所; |
| |
| 摘 要： | 目的探讨人乳头瘤病毒(HPV)免疫层析技术快速检测分型方法的临床应用价值。方法采用免疫层析技术快速检测法对人乳头瘤病毒进行检测分型,采用基因检测技术PCR检测法作对照。结果对386例标本进行HPV常见亚型6、11型和16、18型检测,快速检测法与对照PCR检测法均105例阳性,阳性率均为27.20%,两种方法的阳性率一致(P>0.05)。对HPV亚型总的分型检测,快速检测法检出阳性105例,阳性率为27.20%;对照PCR检测法检出HPV阳性142例,阳性率为36.78%,两种方法的阳性率差异有统计学意义(χ2=8.15,P<0.05)。结论免疫层析快速检测分型方法用于HPV常见亚型6、11型(低危型)和16、18型(高危型)检测,具有操作简便,检测速度快,准确率高,医疗成本较低等特点,值得基层医院推广应用。
|
| 关 键 词： | 人乳头瘤病毒(HPV) 免疫层析技术 分型 聚合酶链反应 |
Clinical application to assure the type of human papillomavirus (HPV) by the rapid detection method |
| |
| LING Yun,FENG Chun-yan,HU Ji-wen,SUN Xing-bao,LUO Yu-xuan,WU Yue-hong,XING Bi-fen,ZHU Cai-yun. Clinical application to assure the type of human papillomavirus (HPV) by the rapid detection method[J]. Journal of Pathogen Biology, 2011, 0(7) |
| |
| Authors: | LING Yun FENG Chun-yan HU Ji-wen SUN Xing-bao LUO Yu-xuan WU Yue-hong XING Bi-fen ZHU Cai-yun |
| |
| Affiliation: | LING Yun1,FENG Chun-yan2,HU Ji-wen2,SUN Xing-bao3,LUO Yu-xuan2,WU Yue-hong1,XING Bi-fen1,ZHU Cai-yun2 (1.Department of Gynaecology and Obstetrics,Longhua People's Hospital of Shenzhen,Shenzhen 518109,Guangdong,China,2.Clinical Laboratory,3.Shenzhen Feipeng Institute of Biology) |
| |
| Abstract: | Objective To study the clinical application to assure the type of human papillomavirus(HPV) by the fast immunochromatography.Methods We assure the type of HPV by the fast immunochromatography.At the same time,the control sample was tested by PCR.Results We detected genotype 6,11and 16,18 from 386 samples by the two methods.The positive rate is same for common genotype 6,11and 16,18(P>0.05).But the total positive rates are different.The positive rate of the immunochromatography method is higher than the PCR.... |
| |
| Keywords: | Human papillomavirus(HPV) immunochromatography genotype PCR |
| 本文献已被 CNKI 等数据库收录！ |
|
