| 不同引物检测甲型H1N1流感病毒的比较研究 |
| |
| 引用本文： | 李忠,韩莹,刘倜,张圣洋,王宇路,王显军,徐爱强,毕振强.不同引物检测甲型H1N1流感病毒的比较研究[J].中国病原生物学杂志,2011(4). |
| |
| 作者姓名： | 李忠 韩莹 刘倜 张圣洋 王宇路 王显军 徐爱强 毕振强 |
| |
| 作者单位： | 山东省疾病预防控制中心;山东省传染病预防控制重点实验室;山东大学预防医学研究院;山东省艾克韦生物技术有限公司; |
| |
| 基金项目： | 山东省科技厅计划项目(No.2009GG10002054) |
| |
| 摘 要： | 目的筛选灵敏、特异的甲型H1N1流感病毒核酸检测方法。方法采用国家流感中心推荐的甲型流感病毒(H1N1)核酸引物和1对自行设计的引物,同时选择市售荧光定量PCR试剂盒,分别对不同浓度的流感病毒进行RT-PCR或荧光定量PCR扩增,比较其检测的灵敏度和特异性。结果荧光定量PCR检测H1N1病毒核酸的灵敏度为10-5(病毒稀释度),高于普通RT-PCR的灵敏度10-3~10-4;RT-PCR扩增甲型流感病毒(H1N1)核酸时,自行设计引物的灵敏度为10-4,高于中国CDC推荐引物的灵敏度10-3。2种引物的特异性一致。结论对于甲型(H1N1)流感病毒疑似样品的检测,荧光定量PCR是较灵敏的方法,但从成本和灵敏度两方面考虑,自行设计的引物更适合基层疾控机构采用。
|
| 关 键 词： | 甲型流感(H1N1) 引物 灵敏度 |
Study on the sensitivity of different primers at detecting nucleic acids of the influenza A H1N1 virus |
| |
| LI Zhong,HAN Yin,LIU Ti,ZHANG Sheng-yang,WANG Yu-lu,WANG Xian-jun,XU Ai-qiang,BI Zhen-qiang.Study on the sensitivity of different primers at detecting nucleic acids of the influenza A H1N1 virus[J].Journal of Pathogen Biology,2011(4). |
| |
| Authors: | LI Zhong HAN Yin LIU Ti ZHANG Sheng-yang WANG Yu-lu WANG Xian-jun XU Ai-qiang BI Zhen-qiang |
| |
| Institution: | LI Zhong1,HAN Yin2,LIU Ti1,ZHANG Sheng-yang1,WANG Yu-lu1,WANG Xian-jun1,XU Ai-qiang1,BI Zhen-qiang1 (1.Shandong Center for Disease Control and Prevention,Key Lab of Infectious Diseases in Shandong Province,Jinan 250014,China,2.Shandong Acv Biotechnologies Co,ltd) |
| |
| Abstract: | Objective To select an assay with high sensitivity and specificity at detecting nucleic acids of the influenza A H1N1 virus.Methods Sensitivity was analyzed using RNA extracted from a 10-fold serially diluted viral culture.Nucleic acids were amplified using fluorescence quantitative PCR and RT-PCR.Two pairs of primer were used for RT-PCR.One pair of primers was designed by the authors and the other was recommended by the Chinese Center for Disease Control and Prevention.Results Fluorescence quantitative PCR... |
| |
| Keywords: | Pandemic A(H1N1) influenza primer sensitivity |
| 本文献已被 CNKI 等数据库收录！ |
|
