| 霍乱毒素联合弓形虫排泄-分泌抗原鼻内免疫小鼠诱导的GALT和NALT黏膜免疫应答 |
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| 引用本文： | 李润花,殷丽天,孟晓丽,刘红丽,王海龙,殷国荣.霍乱毒素联合弓形虫排泄-分泌抗原鼻内免疫小鼠诱导的GALT和NALT黏膜免疫应答[J].中国病原生物学杂志,2011(4). |
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| 作者姓名： | 李润花 殷丽天 孟晓丽 刘红丽 王海龙 殷国荣 |
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| 作者单位： | 太原师范学院生物系;山西医科大学生理学系细胞生理学省部共建教育部重点实验室;山西医科大学寄生虫学教研室; |
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| 基金项目： | 国家自然科学基金项目(No.81071374) |
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| 摘 要： | 目的观察霍乱毒素(CT)联合弓形虫排泄-分泌抗原(ESA)鼻内免疫小鼠诱导的肠相关淋巴组织(GALT)和鼻相关淋巴组织(NALT)黏膜部位的免疫应答。方法将48只5~6周龄BABL/c小鼠随机均分为3组,分别用PBS、ESA和CT+ESA滴鼻免疫小鼠2次,间隔2周。末次免疫后14 d,处死小鼠,ELISA测定小鼠粪便和鼻咽冲洗液sIgA抗体水平;计数派伊尔淋巴集结(PP)、肠上皮淋巴细胞(IEL)、NALT和鼻通道(NC)淋巴细胞数,免疫细胞化学法检测CD4+、CD8+T细胞亚群水平。结果免疫后14 d,CT+ESA组小鼠鼻咽冲洗液和粪便sIgA水平显著高于ESA组和PBS组(P<0.01);CT+ESA和ESA组小鼠GALT部位的PP淋巴细胞显著增生,主要以CD4+T细胞为主;而IEL以CD8+T细胞增生为主,CD4+/CD8+比值降低(P<0.05)。CT+ESA NALT内淋巴细胞明显增生。CT+ESA组NC淋巴细胞显著升高(P<0.01),以CD4+T细胞增生为主。结论 CT佐剂联合ESA滴鼻免疫BALB/c小鼠可诱导GALT和NALT黏膜部位免疫应答。
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| 关 键 词： | 刚地弓形虫 霍乱毒素 排泄-分泌抗原 黏膜免疫 肠相关淋巴组织 鼻相关淋巴组织 |
Intranasal immunization with cholera toxin and excreted-secreted antigens of Toxoplasma gondii induces an immune response at both NALT and GALT mucosal sites in mice |
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| LI Run-hua,YIN Li-tian,MENG Xiao-li,LIU hong-li,WANG Hai-long,YIN Guo-rong..Intranasal immunization with cholera toxin and excreted-secreted antigens of Toxoplasma gondii induces an immune response at both NALT and GALT mucosal sites in mice[J].Journal of Pathogen Biology,2011(4). |
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| Authors: | LI Run-hua YIN Li-tian MENG Xiao-li LIU hong-li WANG Hai-long YIN Guo-rong |
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| Institution: | LI Run-hua1,YIN Li-tian2,MENG Xiao-li3,LIU hong-li3,WANG Hai-long3,YIN Guo-rong3.(1.Department of Biology,Taiyuan Normal University,Taiyuan 030031,China,2.Department of Physiology,Key Laboratory of Cellular Physiology constructed jointly by Shanxi Province and the Ministry of Education,Shanxi Medical University,3.Department of Parasitology,Shanxi Medical University) |
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| Abstract: | |
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| Keywords: | Toxoplasma gondii cholera toxin excreted-secreted antigens mucosal immunity gut associated lymphoid tissue(GALT) nasal associated lymphoid tissue(NALT) |
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