| 细粒棘球绦虫EgAgB8/1重组抗原和EgAgB8/1-EgAgB8/2重组嵌合抗原的表达及血清学诊断价值的比较 |
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| 引用本文： | 古力帕丽·麦曼提依明,马海梅,吾拉木·马木提,陈洁,陈璐,丁剑冰,马秀敏. 细粒棘球绦虫EgAgB8/1重组抗原和EgAgB8/1-EgAgB8/2重组嵌合抗原的表达及血清学诊断价值的比较[J]. 中国病原生物学杂志, 2011, 0(6) |
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| 作者姓名： | 古力帕丽·麦曼提依明 马海梅 吾拉木·马木提 陈洁 陈璐 丁剑冰 马秀敏 |
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| 作者单位： | 新疆医科大学基础医学院寄生虫学教研室;新疆医科大学第一附属医院包虫病重点实验室; |
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| 基金项目： | 国家自然科学基金项目(No.30760229); 新疆维吾尔自治区高校科研计划项目(No.XJEDU2008S31) |
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| 摘 要： | 目的诱导表达并纯化细粒棘球绦虫EgAgB8/1重组抗原和EgAgB8/1-EgAgB8/2重组嵌合抗原,比较两个重组蛋白对囊型包虫病(CE)的血清学诊断价值。方法 IPTG诱导转染至E.coliBL21(DE3)LysS的pET32a-EgAgB8/1和pET32a-EgAgB8/1-EgAgB8/2重组原核表达质粒,表达和纯化EgAgB8/1重组蛋白和EgAgB8/1-EgAgB8/2重组嵌合蛋白,用SDS-PAGE电泳分析鉴定重组蛋白,并通过超声裂解法进行纯化,以CE病人及囊虫病人血清为一抗,Western blot法检测两个重组蛋白免疫反应性。结果细粒棘球绦虫重组抗原pET32a-EgAgB8/1和pET32a-EgAgB8/1-EgAgB8/2重组原核表达质粒得到成功诱导表达,经SDS-PAGE电泳分析,重组蛋白分子质量单位为28 ku和38 ku;Western blot结果表明,EgAgB8/1重组蛋白和EgAgB8/1-EgAgB8/2重组嵌合蛋白均能被CE病人血清特异性识别,16份CE病人血清中,以EgAgB8/1重组蛋白为抗原时11份阳性,以EgAgB8/1-EgAgB8/2重组蛋白为抗原时...
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| 关 键 词： | 细粒棘球绦虫 EgAgB8/1重组抗原 EgAgB8/1-EgAgB8/2重组嵌合抗原 血清学诊断 |
Comparison of the expression and serological evaluation of the recombinant protein EgAgB8/1 and the recombinant chimeric protein EgAgB8/1-EgAgB8/2 from Echinococcus granulosus |
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| MAIMANTIYIMING Gulipali,,MA Hai-mei,MAMUTI Wulamu,CHEN Jie,CHEN Lu,DING Jian-bing,MA Xiu-min. Comparison of the expression and serological evaluation of the recombinant protein EgAgB8/1 and the recombinant chimeric protein EgAgB8/1-EgAgB8/2 from Echinococcus granulosus[J]. Journal of Pathogen Biology, 2011, 0(6) |
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| Authors: | MAIMANTIYIMING Gulipali MA Hai-mei MAMUTI Wulamu CHEN Jie CHEN Lu DING Jian-bing MA Xiu-min |
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| Affiliation: | MAIMANTIYIMING Gulipali1,2,MA Hai-mei2,MAMUTI Wulamu1,CHEN Jie1,CHEN Lu1,DING Jian-bing2,MA Xiu-min2 (1.Department of Parasitology of the School of Basic Medical Science,Xinjiang Medical University,Urumqi 830054,China,2.Hydatid Disease Key Laboratory,the first Affiliated Hospital of Xinjiang Medical University) |
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| Abstract: | Objective To express and serologically evaluate the recombinant protein EgAgB8/1 and the recombinant chimeric protein EgAgB8/1-EgAgB8/2 from Echinococcus granulosus in order to serologically diagnose cystic echinococcus(CE).Methods The recombinant prokaryotic expression vectors pET32a-EgAgB8/1 and pET32a-EgAgB8/1-EgAgB8/2 were introduced into E.coli BL21(DE3) LysS and induced with IPTG to express protein.The recombinant proteins obtained were purified by ultrasonication.The serological reactivity of these r... |
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| Keywords: | Echinococcus granulosus recombinant protein EgAgB8/1 recombinant chimeric protein EgAgB8/1-EgAgB8/2 serological diagnosis |
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