| 实时荧光定量RT-PCR检测急性出血性结膜炎CoxA24v病毒 |
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| 引用本文： | 李洁,黄芳,郭婧,崔淑娟,贾蕾,李锡太,黎新宇,王全意.实时荧光定量RT-PCR检测急性出血性结膜炎CoxA24v病毒[J].中国病原生物学杂志,2011(6). |
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| 作者姓名： | 李洁 黄芳 郭婧 崔淑娟 贾蕾 李锡太 黎新宇 王全意 |
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| 作者单位： | 北京市疾病预防控制中心传染病地方病控制所; |
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| 摘 要： | 目的对检测急性出血性结膜炎(AHC)病原CoxA24v的实时荧光定量RT-PCR方法进行特异性、敏感性、稳定性等方面的评估。方法利用实时荧光定量RT-PCR检测肠道病毒CoxA24v和其他几种肠道病毒,验证方法的特异性;根据TCID50进行倍比稀释,验证该方法的敏感性;将样品进行倍比稀释,做重复试验,验证方法的准确性。利用实时荧光定量RT-PCR对疑似AHC患者眼拭子进行检测。结果该方法对CoxA24v病毒的检测具有高度的特异性,对柯萨奇病毒A16、肠道病毒71等其他肠道病毒均无交叉反应。检测的灵敏度达0.1TCID50。用该方法对6个不同浓度的CoxA24 v进行5次检测,获得的结果重复性好。采用该方法检测疑似AHC患者标本,阳性者均测序,证明是Cox-A24v,未出现假阳性。结论实时荧光定量RT-PCR法是一种快速、敏感、特异、准确的方法,适用于急性出血性结膜炎的早期诊断和鉴别诊断。
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| 关 键 词： | 实时荧光定量RT-PCR CoxA24v 急性出血性结膜炎 |
Rapid detection of CoxA24v from acute hemorrhagic conjunctivitis by real-time RT-PCR |
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| LI Jie,HUANG Fang,GUO Jing,CUI Shu-juan,JIA Lei,LI Xi-tai,LI Xin-yu,WANG Quan-yi.Rapid detection of CoxA24v from acute hemorrhagic conjunctivitis by real-time RT-PCR[J].Journal of Pathogen Biology,2011(6). |
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| Authors: | LI Jie HUANG Fang GUO Jing CUI Shu-juan JIA Lei LI Xi-tai LI Xin-yu WANG Quan-yi |
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| Institution: | LI Jie,HUANG Fang,GUO Jing,CUI Shu-juan,JIA Lei,LI Xi-tai,LI Xin-yu,WANG Quan-yi (Institute for Infectious Disease and Endemic Disease Control,Beijing Center for Disease Prevention and Control,Beijing 100013,China) |
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| Abstract: | Objective To further investigate the specificity,sensitivity,and accuracy of a established method of real-time RT-PCR to detect coxsackievirus A24 variant(CoxA24v),a cause of acute hemorrhagic conjunctivitis(AHC).Methods The specificity of the method was evaluated by detecting CoxA24v and several other enteroviruses.The CoxA24v strain was diluted in accordance with its TCID50 and the sensitivity of real-time RT-PCR was evaluated.The experiment was repeated and accuracy of the assay was evaluated.Clinical sp... |
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| Keywords: | Real-time RT-PCR coxsackievirus A24 variant acute hemorrhagic conjunctivitis |
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