ConA增强NФ吞噬功能、杀瘤效应及产生效应分子的研究

目的了解 Con A在体内对小鼠腹腔 MΦ 吞噬功能、细胞毒作用及产生 NO、TNF- α、IL- 1的影响。方法分别测MΦ 吞噬鸡红细胞的能力 ,以 S180为靶细胞测 MΦ 依赖的细胞毒作用。以 griess试剂、L92 9细胞 MTT检测法、胸腺细胞增殖法测 MΦ 产生 NO、TNF- α、IL- 1的水平。结果 Con A活化的腹腔 MΦ 吞噬鸡红细胞的能力以及对 S180细胞的细胞毒作用显著强于 PBS对照组 (P<0 .0 1) ,并产生较高水平的 NO、TNF- α、IL- 1。结论 Con A能活化 MΦ 产生 NO等效应分子 ,发挥对 MΦ的免疫调节作用

Study on ConA promoting phagocytosis, cytotoxicity and producing effectors of macrophages

Objective　To study　how　ConA actives macrophages in vivo to produce cytotoxic effectors and its phagocytic functions,and　 cytotoxicity. Methods　 ConA was intraperitoneally injected(ip). Cock red blood cells(cRBC) were used to evaluate MΦ phagocytic activity,and S180 cells as target cells to analyze MΦ dependent cytotoxicity(MTC).Nitric oxide(NO),TNF-α　and IL-1 levels of MΦ cultural supernatant were measured using griess reagent,L929 cells MTT method and thymocytes proliferation test respectively. Results　 ConA could promote MΦ　to phagocytize cRBC and kill S180 cells,enhance the production of such factors as NO,TNF-α　and IL-1 by MΦ. There was significant difference compared with PBS　control group(P＜0.01). Conclusions　 ConA could stimulate MΦ to produce effectors, which mediate immune regulation of ConA to MΦ.