Applied research on serum protein fingerprints for prediction of Qi deficiency syndrome and phlegm and blood stasis in patients with non-small cell lung cancer |
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Authors: | Zhizhen Liu Jian Du Academy of Integrative Medicine of Fujian University of TCM Fuzhou Fujian China Zongyang Yu Xuenong OuYang |
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Affiliation: | ,Department of Oncology,Fuzhou General Hospital,Nanjing Military Area,Fuzhou 350025,China Xiaopeng Lan,Meng Zhao,PLA Medical Laboratory Center of Fuzhou General Hospital,Fuzhou 350025,China |
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Abstract: | OBJECTIVE:This study screened serum tumor biomarkers by surface enhanced laser desorption/ionization time-of-flight mass spectrometry(SELDI-TOF-MS) to establish a subset which could be used for the prediction of Qi deficiency syndrome and phlegm and blood stasis in patients with non-small cell lung cancer;and as diagnostic model of Chinese medicine.METHODS:Serum samples from 63 lung cancer patients with Qi deficiency syndrome and phlegm and blood stasis,and 28 lung cancer patients with non-Qi deficiency syndrome and phlegm and blood stasis were analyzed using SELDI-TOF-MS with a PBS II-C protein chip reader.Protein profiles were generated using immobilized metal affinity capture(IMAC3) protein chips.Differentially-expressed proteins were screened.Protein peak clustering and classification analyses were performed using Biomarker Wizard and Biomarker Pattern software packages,respectively.RESULTS:A total of 268 effective protein peaks were detected in the 1,000-10,000 Da molecular range for the 15 serum proteins screened(P<0.05).The decision tree model was M 2284.97,with a sensitivity of 96.2% and a specificity of 66.7%.CONCLUSION:SELDI-TOF-MS techniques,combined with a decision tree model,can help identify serum proteomic biomarkers related to Qi deficiency syndrome and phlegm and blood stasis in lung cancer patients;and the predictive model can be used to discriminate between Chinese medicine diagnostic models of disease. |
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Keywords: | Lung neoplasms Deficiency symptomcomplex Intermingled phlegm and blood-stasis Spectrometry,Mass,Matrix-assisted laser desorption-Ionization Proteomics Computational biology Protein array analysis |
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