| O157血清群大肠杆菌分子亚型的研究 |
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| 引用本文： | 毕振强,崔树玉,孙启华,温宪芹,孟蔚,关冰,郭维植,陈亢川.O157血清群大肠杆菌分子亚型的研究[J].海峡预防医学杂志,2002,8(4):10-12. |
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| 作者姓名： | 毕振强 崔树玉 孙启华 温宪芹 孟蔚 关冰 郭维植 陈亢川 |
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| 作者单位： | 1. 山东省卫生防疫站,济南,250014
2. 福建省卫生防疫站,福州,350001 |
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| 摘 要： | 目的：了解山东和福建两省不同来源的O157血清群大肠杆菌分子亚型和菌株间的相关性。方法：对1993－1996年间分离到菌株进行脉冲场电泳（PFGE），图象经计算机处理后进行聚类分析。结果：O157大肠杆菌染色体DNA用XbaI酶切后，经PFGE可生15－20条带，以90％的同源性作为界值，可将11株菌分成9个PFGE亚型，其中山东分离到的6株菌分成4个亚型，福建的5株菌分属5个亚型。结论：山东、福建的O157血清群大肠杆菌均存在多个克隆系。
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| 关 键 词： | O157大肠杆菌 分子亚型 同源性 脉冲场电泳 |
| 文章编号： | 1007-2705(2002)04-0010-03 |
| 修稿时间： | 2001年11月5日 |
Study on Molecular Subtypes of Escherichia Coli O157 Serogroup by Pulsed-Field Electrophoresis |
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| BI Zhen qiang,CUI Shu yu,SUN Qi hua,et al.Study on Molecular Subtypes of Escherichia Coli O157 Serogroup by Pulsed-Field Electrophoresis[J].Strait Journal of Preventive Medicine,2002,8(4):10-12. |
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| Authors: | BI Zhen qiang CUI Shu yu SUN Qi hua |
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| Abstract: | To study the correlation of molecular subtypes and strains of Escherichia coli O157 (E.coli O157) isolated from Shandong and Fujian, China. The pulsed field gel electrophoresis (PFGE) was applied to the O157 strains isolated from 1993 to 1996. And the images were processed by cluster analysis using computer software. Digestion of chromosomal DNA with Xba I yielded 15 to 20 bands. With 90% homology as cutoff value, 11 strains of O157 isolates were classified into 9 PFGE types, among which 6 isolates from Shandong classified into 4 PFGE type, 5 strains from Fujian classified into 5 different subtypes.Conclusion] There exists multi clone of E.coli O157 in both Shangdong and Fujian, China. |
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| Keywords: | Escherichia coli O157 molecular subtype homology PFGE |
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