| 针对乙酰肝素酶基因的短发夹RNA干扰载体的构建 |
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| 引用本文： | 许立薇,刘阳,霍强,王爱红,王启光.针对乙酰肝素酶基因的短发夹RNA干扰载体的构建[J].新乡医学院学报,2011,28(1):7-9. |
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| 作者姓名： | 许立薇 刘阳 霍强 王爱红 王启光 |
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| 作者单位： | 1. 蚌埠医学院药学系,安徽,蚌埠,233030;安徽省生化药物工程技术研究中心,安徽,蚌埠,233030
2. 安徽省生化药物工程技术研究中心,安徽,蚌埠,233030;安徽宏业药业有限公司质量管理部,安徽,蚌埠,233030 |
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| 基金项目： | 安徽省高等学校省级自然科学研究项目(编号:KJ2009B150Z); 蚌埠医学院科研项目(编号:BY0822) |
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| 摘 要： | 目的 构建对人乙酰肝素酶(hpa)基因有特异性抑制作用的短发夹RNA(ahRNA)表达载体.方法 根据GenBank数据库提供的hpa基因核苷酸序列,按照shRNA设计原则设计5对特异性的寡核苷酸序列及阴性对照,分别克隆到pGPU6/GFP/Neo载体中,酶切鉴定和测序验证阳性克隆.结果 经酶切、测序鉴定均证实重组质粒...
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| 关 键 词： | 乙酰肝素酶 RNA干扰 短发夹RNA |
Construction of short hairpin RNA vector targeted to heparitinase gene |
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| XU Li-wei,LIU Yang,HUO Qiang,WANG Ai-hong,WANG Qi-guang.Construction of short hairpin RNA vector targeted to heparitinase gene[J].Journal of Xinxiang Medical College,2011,28(1):7-9. |
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| Authors: | XU Li-wei LIU Yang HUO Qiang WANG Ai-hong WANG Qi-guang |
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| Institution: | XU Li-wei1,2,LIU Yang1,HUO Qiang1,WANG Ai-hong2,3,WANG Qi-guang2,3 (1.Pharmacy Department of Bengbu Medical College,Bengbu 233030,Anhui Province,China,2.Technique Research Center for Biochemical Pharmaceutical Engineering,3.Quality Management Department of Anhui Hongye Medicine Company,China) |
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| Abstract: | Objective To construct expression vectors of short hairpin RNA(shRNA) having specific inhibition for heparitinase(hpa) gene.Methods According to nucleotide sequences of hpa gene supplied from GenBank database,five pairs of specific interference sequences and negative control were designed with shRNA principle of design,and were cloned into the vectors of pGPU6/GFP/Neo respectively.Then the masculine clones were confirmed by restriction enzyme digestion and sequencing analysis.Results Both restriction enzyme... |
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| Keywords: | heparitinase RNA interference short hairpin RNA |
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