| 一种适用于膜片钳研究的豚鼠心室肌细胞急性分离方法 |
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| 引用本文： | 张艳,董献红,李新强,李晓娟,李东亮.一种适用于膜片钳研究的豚鼠心室肌细胞急性分离方法[J].新乡医学院学报,2011,28(1):14-16. |
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| 作者姓名： | 张艳 董献红 李新强 李晓娟 李东亮 |
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| 作者单位： | 1. 新乡医学院基础医学院生理学与神经生物学教研室,河南,新乡,453003
2. 新乡医学院基础医学院药理学教研室,河南,新乡,453003 |
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| 基金项目： | 河南省教育厅自然科学研究资助项目(编号:2008A180028) |
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| 摘 要： | 目的 获得适用于心肌细胞膜片钳研究的单个心室肌细胞.方法 应用酶消化和机械分离的方法分离出生25～30 d的豚鼠心室肌细胞,从形态学及电生理学方面鉴定急性分离的豚鼠心室肌细胞活性,在电镜下观察活性较好的豚鼠心室肌细胞形态学特点,并选用活性较好的单个心室肌细胞进行膜片钳钳制,记录心室肌细胞Ik电流.结果 形态学方面:活性...
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| 关 键 词： | 豚鼠 心室肌细胞 全细胞膜片钳技术 延迟整流钾电流 |
One method for patch-clamp study in acutely isolated guinea pig ventricular myocytes |
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| ZHANG Yan,DONG Xian-hong,LI Xin-qiang,LI Xiao-juan,LI Dong-liang.One method for patch-clamp study in acutely isolated guinea pig ventricular myocytes[J].Journal of Xinxiang Medical College,2011,28(1):14-16. |
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| Authors: | ZHANG Yan DONG Xian-hong LI Xin-qiang LI Xiao-juan LI Dong-liang |
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| Institution: | ZHANG Yan1,DONG Xian-hong1,LI Xin-qiang2,LI Xiao-juan1,LI Dong-liang1 (1.Department of Physiology and Neurobiology,Xinxiang Medical College,Xinxiang 453003,Henan Province,China,2.Department of Pharmacology,China) |
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| Abstract: | Objective To obtain the single ventricular myocytes which were fitted the study of whole-cell patch-clamp.Methods Single ventricular myocytes of guinea pigs(25-30 days) were isolated with the collagenase dispersion and mechanical divorced methods,and cytoactive was indentified with morphology and electrophysiology methods.The morphology feature of single ventricular myocytes of guinea pigs was observed with electron microscope.The better cytoactive of single ventricular myocytes were adopted to patch clamp ... |
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| Keywords: | guinea pig ventricular myocytes whole-cell patch-clamp delayed rectifier potassium current |
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