| 3种肺炎支原体感染检测方法的临床效果比较 |
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| 引用本文： | 王佳贺,牛慧彦,白雪,李岩,何平.3种肺炎支原体感染检测方法的临床效果比较[J].新乡医学院学报,2011,28(1):51-53. |
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| 作者姓名： | 王佳贺 牛慧彦 白雪 李岩 何平 |
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| 作者单位： | 中国医科大学附属盛京医院老年病科,辽宁沈阳,110004 |
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| 基金项目： | 辽宁省教育厅资助项目(编号:L2010701) |
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| 摘 要： | 目的 比较实时荧光定量聚合酶链反应(FQ-PCR)法、培养-增强套式PCR(培养-增强nPCR)法和培养法检测肺炎支原体感染的临床应用价值.方法 分别采用实时FQ-PCR法、培养-增强nPCR法和培养法对临床诊断为肺炎支原体肺炎和疑似肺炎支原体肺炎的97例患儿的咽拭子标本进行检测.结果 实时FQ-PCR法检测的阳性率明...
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| 关 键 词： | 肺炎支原体 实时荧光定量聚合酶链反应 套式聚合酶链反应 培养法 |
Comparison of three methods for detection of Mycoplasma pneumonia |
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| WANG Jia-he,NIU Hui-yan,BAI Xue,LI Yan,HE Ping.Comparison of three methods for detection of Mycoplasma pneumonia[J].Journal of Xinxiang Medical College,2011,28(1):51-53. |
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| Authors: | WANG Jia-he NIU Hui-yan BAI Xue LI Yan HE Ping |
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| Institution: | WANG Jia-he,NIU Hui-yan,BAI Xue,LI Yan,HE Ping (Department of Geriatrics,Shengjing Hospital Affiliated to China Medical University,Shenyang 110004,Liaoning Province,China) |
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| Abstract: | Objective To compare the clinical application value of real-time fluorescence quantitation polymerase chain reaction(FQ-PCR),cultured-enhanced nest PCR(nPCR) and mycoplasma culture in the detection of Mycoplasma pneumonia.Methods Pharyngeal swab specimen from 97 children(Mycoplasma pneumonia patients or Mycoplasma pneumonia-suspected patients)was detected by means of real-time FQ-PCR,cultured-enhanced nPCR and mycoplasma cultivation.Results The positive rate of detection with real-time FQ-PCR was significan... |
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| Keywords: | Mycoplasma pneumonia real-time fluorescent quantitation polymerase chain reaction nest polymerase chain reaction cultivation |
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