| 固相萃取HPLC-UV法测定人血浆中比阿培南浓度及其药代动力学研究(英文) |
| |
| 引用本文： | 朱旭,孙亚欣,何晓静,邱枫,李岭,肇丽梅. 固相萃取HPLC-UV法测定人血浆中比阿培南浓度及其药代动力学研究(英文)[J]. 中国药学, 2011, 20(1): 70-76. DOI: 10.5246/jcps.2011.01.010 |
| |
| 作者姓名： | 朱旭 孙亚欣 何晓静 邱枫 李岭 肇丽梅 |
| |
| 作者单位： | 中国医科大学附属盛京医院药学部;中国医科大学基础医学院医学遗传学教研室;东北大学中荷生物医学与信息工程学院; |
| |
| 基金项目： | National Natural Science Foundation of China (Grant No.30973597) |
| |
| 摘 要： | 比阿培南是一种新型经胃肠外吸收的碳青霉烯类抗生素,广泛用于治疗多种细菌感染。本试验建立了一种简便、有效、精确的固相萃取HPLC法测定人血浆中比阿培南的浓度。样品和内标物Vitamin B6经固相萃取后通过Capcell Pak C18柱进行分离。测定方法以0.05mol/L磷酸二氢钠(pH5.7)–甲醇(98:2,v/v)为流动相,流速为1.0mL/min,检测波长为300nm。比阿培南在0.04~50.00μg/mL浓度范围内线性关系良好,最低定量限可至0.04μg/mL,0.10、5.00和25.00μg/mL三个浓度的提取回收率均在70%左右。这种测定方法成功地应用于12名健康志愿者单次静脉滴注三剂量(150、300和600mg)比阿培南后的药动学研究。本方法具有良好的精密度和准确度,并且保证样品处理后的稳定性,因此能广泛应用于临床研究。
|
| 关 键 词： | 比阿培南 固相萃取 高效液相色谱法 药代动力学 |
HPLC-UV method with solid-phase extraction for the quantitative determination of biapenem in human plasma and its application in pharmacokinetic study |
| |
| Xu Zhu ,Ya-Xin Sun ,Xiao-Jing He ,Feng Qiu ,Jesse Ling Li ,Li-Mei Zhao. HPLC-UV method with solid-phase extraction for the quantitative determination of biapenem in human plasma and its application in pharmacokinetic study[J]. Journal of Chinese Pharmaceutical Sciences, 2011, 20(1): 70-76. DOI: 10.5246/jcps.2011.01.010 |
| |
| Authors: | Xu Zhu Ya-Xin Sun Xiao-Jing He Feng Qiu Jesse Ling Li Li-Mei Zhao |
| |
| Affiliation: | Xu Zhu 1,Ya-Xin Sun 1,Xiao-Jing He 1,Feng Qiu 1,Jesse Ling Li 2,3,Li-Mei Zhao 1* 1. Department of Pharmacy,Shengjing Hospital of China Medical University,Shenyang 110004,China 2. Department of Medical Genetics,China Medical University,Shenyang 110001,China 3. Sino-Dutch Biomedical and Information Engineering School,Northeastern University,Shenyang 110003,China |
| |
| Abstract: | Biapenem, a new parenteral carbapenem, has been widely used for treating bacterial infections. A simple, effective and accurate method based on solid-phase extraction (SPE) and HPLC was developed for the quantitative determination of biapenem in human plasma. Stability and feasibility of the method was validated through a series of experiments. Using Vitamin B 6 asan internal standard, analyte was separated on a Capcell Pak C 18 column after SPE on Oasis hydrophilic-lipophilic balance (HLB) cartridge. The m... |
| |
| Keywords: | Biapenem Solid-phase extraction High-performance liquid chromatography Pharmacokinetic study |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《中国药学》浏览原始摘要信息 |
|
点击此处可从《中国药学》下载免费的PDF全文 |
|
