| 高效液相色谱法测定虫草安肺片中盐酸麻黄碱含量 |
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| 引用本文： | 费超,孟蕾,张海鸣,曹杰,刘子沭,杜树山.高效液相色谱法测定虫草安肺片中盐酸麻黄碱含量[J].中国中医药信息杂志,2011,18(1):57-58. |
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| 作者姓名： | 费超 孟蕾 张海鸣 曹杰 刘子沭 杜树山 |
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| 作者单位： | 1. 中药资源保护与利用北京市重点实验室,北京,100875
2. 北京师范大学校医院,北京,100875 |
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| 基金项目： | 北京市科委重大培育专项(Y0204003041011) |
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| 摘 要： | 目的建立虫草安肺片中盐酸麻黄碱的含量测定方法。方法采用高效液相色谱法，选用Diamonsi1C18色谱柱（4．6mm×250mm，5μm），以乙腈-0．025mol／L磷酸水溶液（5：95）为流动相，流速1．0mL／min，柱温30℃，检测波长为210nm。结果盐酸麻黄碱在1．950～5．850μg范围内呈现良好的线性关系（r=0．9999），平均回收率为99．08％，RSD=3．01％，拖尾因子为1．03。结论本方法快速、简便、准确、重复性较好，结果可靠，可用于控制虫草安肺片制剂的质量。
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| 关 键 词： | 虫草安肺片 盐酸麻黄碱 高效液相色谱法 含量测定 |
Determination of Ephedrine Hydrochloride in Chongcaoanfei Tablets by HPLC |
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| FEI Chao,MENG Lei,ZHANG Hai-ming,CAO Jie,LIU Zi-mu,DU Shu-shan.Determination of Ephedrine Hydrochloride in Chongcaoanfei Tablets by HPLC[J].Chinese Journal of Information on Traditional Chinese Medicine,2011,18(1):57-58. |
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| Authors: | FEI Chao MENG Lei ZHANG Hai-ming CAO Jie LIU Zi-mu DU Shu-shan |
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| Institution: | FEI Chao1,MENG Lei2,ZHANG Hai-ming1,CAO Jie1,LIU Zi-mu1,DU Shu-shan1(1.Beijing Area Major Laboratory of Protection and Utilization of Traditional Chinese Medicine,Beijing 100875,China,2.School Hospital of Beijing Normal University,China) |
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| Abstract: | Objective To establish an accurate method for the determination of Ephedrine hydrochloride in Chongcaoanfei Tablets. Methods HPLC method was carried on Diamonsil C18 column （4.6 mm × 250 mm, 5 μm） with a mobile phase consisting of acetonitri/e-0.025 mol/L phosphate （5 ： 95）, the flow rate was 1.0 mL/min and column temperature was 30 ℃. The detection wavelength was 210 nm. Results The linear range of Ephedrine hydrochloride was 1.950-5.850μg （r=0.999 9） and the tailing factor was 1.03. The average recovery was 99.08% and RSD was 3.01%. Conclusion The method is simple and accurate, and can be used for the quality control of Chongcaoanfei tablets. |
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| Keywords: | Chongcaoanfei Tablets Ephedrine hydrochloride HPLC content determination |
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