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甲基叔丁基醚对原癌基因和功能基因表达的影响
引用本文:周伟,黄关麟,张恒,叶舜华.甲基叔丁基醚对原癌基因和功能基因表达的影响[J].卫生研究,1999,28(3):137-138.
作者姓名:周伟  黄关麟  张恒  叶舜华
作者单位:1. 上海医科大学环境卫生学教研室,上海,200032
2. 南京炼油厂职工医院
摘    要:甲基叔丁基醚(MTBE)是一种新型的汽油添加剂,被用来提高汽油燃烧效率,减少汽车尾气中有害物质的排放。MTBE具有一定的动物致癌性,但其机制目前并不清楚。本研究采用免疫组织化学方法,检测了MTBE对体外培养的NIH3T3细胞中c-myc和p21蛋白表达的影响;采用点杂交方法,从RNA水平检测了MTBE亚慢性染毒大鼠肝组织中原癌基因c-myc基因和功能基因GST-P基因的表达情况。免疫组化结果显示,MTBE可诱导c-myc基因的高表达,对p21蛋白的表达未见明显影响。点杂交结果显示,MTBE可明显诱导大鼠肝组织中c-myc基因的高表达,而对GST-P基因的表达未见明显影响。上述结果提示,MTBE可诱导细胞中c-myc基因表达活性增高,可能是其动物致癌性的机制之一。

关 键 词:甲基叔丁基醚  c-myc基因  p21蛋白  GST-P基因

Effect of methyl tertiary butyl ether on the expression of protooncogenes and function genes
Zhou Wei,Huang Guanlin,\ Zhangheng et al.Effect of methyl tertiary butyl ether on the expression of protooncogenes and function genes[J].Journal of Hygiene Research,1999,28(3):137-138.
Authors:Zhou Wei  Huang Guanlin  \ Zhangheng
Institution:Department of Environmental Health, Shanghai Medical University, Shanghai 200032, China.
Abstract:Methyl tertiary butyl ether (MTBE) is a new gasoline additive, which is used to increase the combustion of gasoline and to reduce the emission of harmful exhausts from automobile. The mechanism for the carcinogenesis of MTBE in animals is not clear.Immunohistochemistry method was used to detect the effect of MTBE on the expression of c myc and p21 proteins in NIH 3T 3 cells. Dot hybridization method was used to explore the expression of c myc gene and GST P(glutathione S transferase P) gene in the of MTBE treated rats.The results showed that MTBE could enhance the expression of c myc protein,but had no effect on p21 protein. MTBE could induce high expression of c myc gene,and had no effect on the expression of GST P gene.These results suggest that the high expression of c myc gene induced by MTBE might be one of the mechanisms of its carcinogenicity in animal.
Keywords:Methyl tertiary butyl ether  c myc gene  p21 protein  GST P gene  
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