| 高效液相色谱法测定延肾胶囊中淫羊藿苷含量简 |
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| 引用本文： | 王芳,石悦,曹健.高效液相色谱法测定延肾胶囊中淫羊藿苷含量简[J].中国药业,2014(4):37-38. |
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| 作者姓名： | 王芳 石悦 曹健 |
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| 作者单位： | 中国人民解放军第三军医大学新桥医院药学部,重庆400037 |
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| 摘 要： | 目的 建立测定延肾胶囊中淫羊藿苷含量的高效液相色谱法.方法 采用Hypersil ODS色谱柱（4.6mm×150 mm,5μm）,流动相为乙腈-水（25∶75）,检测波长为270 nm,流速1.0 mL/min,柱温25℃.结果 淫羊藿苷质量浓度在12.0 ～ 28.0 μg/mL范围内与峰面积线性关系良好,r =0.999 8（n=5）;平均回收率为99.50％,RSD=1.38％ （n =6）.结论 该方法重现性好、简便易行,可用于延肾胶囊的质量控制.
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| 关 键 词： | 高效液相色谱法 延肾胶囊 淫羊藿苷 含量测定 |
Determination of Icariin in Yanshen Capsules by HPLC |
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| Wang Fang,Shi Yue,Cao Jian.Determination of Icariin in Yanshen Capsules by HPLC[J].China Pharmaceuticals,2014(4):37-38. |
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| Authors: | Wang Fang Shi Yue Cao Jian |
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| Institution: | ( Department of Pharmacy, Xinqiao Hospital, Third Military Medical University, Chongqing, China 400037) |
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| Abstract: | Objective To establish a HPLC method for the determination of the icariin content in Yanshen Capsules.Methods The Hypersil ODS column （150 mm×4.6 mm,5 μm） was used,the mobile phase was acetonitrile-water（25∶75）,the detection wavelength was 270 nm,the flow rate was 1.0 mL/min and the column temperature was 25℃.Results The linear range of paeoniflorin was 12.0～28.0 μg/mL,r=0.999 8（n=5）.The average recovery rate was 99.50%,RSD 1.38% （n=6）.Conclusion This method has good repeatability and is simple,convenient and easy to operate,which can be used for the quality control of Yanshen Capsules. |
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| Keywords: | HPLC Yanshen Capsules icariin content determination |
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