牡荆苷对骨髓间充质干细胞成脂分化的影响简

目的 探讨牡荆苷对大鼠骨髓间充质干细胞成脂分化的影响.方法 采用细胞的增殖检测（MTT）法检测骨髓间充质干细胞生存率,油红O染色检测细胞成脂分化,实时荧光定量PCR检测过氧化物酶体增殖物激活受体γ（PPARγ）和CCAAT增强子结合蛋白α（C/EBPα） mRNA表达.结果 牡荆苷浓度在1～50 μmol/L范围内对骨髓间充质干细胞生存率无显著影响;牡荆苷可抑制骨髓间充质干细胞成脂分化及PPARγ和C/EBPαmRNA表达.结论 牡荆苷可抑制骨髓间充质干细胞成脂分化,其作用机制可能与抑制PPARγ和C/EBPα基因表达有关.

Effect of Vitexin on Adipogenic Differentiation of Bone Marrow Mesenchymal Stem Cells

Objective To investigate the effect of vitexin on the adipogenic differentiation of rat bone marrow mesenchymal stem cells （MSCs）.Methods The methylthiazolyl tetrazolium （MTT） assay was applied to determine the viability of MSCs.The adipogenic differentiation was analyzed by the oil red O staining.The genes expression of peroxisome proliferator activated receptor γ （PPARγ） and CCAAT enhancer-binding protein α （C/EBPα） were detected by the real time PCR.Results Vitexin in the concentration range of 1-50 μmol/L had no significant effect on the viability of MSCs.Vitexin could inhibit the adipogenic differentiation and the expression of PPARγ and EBPα mRNA in MSCs.Conclusion Vitexin can inhibit the adipogenic differentiation of MSCs,which mechanism might be related with the inhibition of PPARγ and EBPα gene in MSCs.