| 高效液相色谱法测定氯锌油中氯霉素含量简 |
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| 引用本文： | 胡娟妮,聂平,邓永琪,李晓燕,李文莉.高效液相色谱法测定氯锌油中氯霉素含量简[J].中国药业,2014(4):44-45. |
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| 作者姓名： | 胡娟妮 聂平 邓永琪 李晓燕 李文莉 |
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| 作者单位： | [1]中南大学,湖南长沙410013 [2]湖南省食品药品检验研究院,湖南长沙410001 |
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| 摘 要： | 目的 建立测定氯锌油中氯霉素含量的高效液相色谱法.方法 采用Waters C18色谱柱（250 mm×4.6 mm,5μm）,流动相为0.01 mol/L庚烷磺酸钠缓冲溶液-甲醇（60∶40）,流速为1.0 mL/min,检测波长为277 nm,柱温为30℃.结果 氯霉素的质量浓度在0.01～1.00 g/L范围内与峰面积线性关系良好,平均回收率为100.18％,RSD=1.38％.结论 方法灵敏度高、操作简便、准确可靠,可用于氯锌油中氯霉素的含量测定.
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| 关 键 词： | 氯锌油 氯霉素 高效液相色谱法 |
Determination of Chloramphenicol Content in Chlorine Zinc Oil by HPLC |
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| Hu Juanni,Nie Ping,Deng Yongqi,Li Xiaoyan,Li Wenli.Determination of Chloramphenicol Content in Chlorine Zinc Oil by HPLC[J].China Pharmaceuticals,2014(4):44-45. |
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| Authors: | Hu Juanni Nie Ping Deng Yongqi Li Xiaoyan Li Wenli |
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| Institution: | 1. Central South University, Changsha, Hunan, China 410013; 2. Hunan Provincial Institute For Food and Drug Control, Changsha, Hunan, China 410001 ) |
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| Abstract: | Objective To establish a method for determining the content of chloramphenicol in chlorine zinc oil.Methods The Waters C18 column（250 mm×4.6 mm,5 μm） was adopted.The mobile phase consisted of 0.01 mol/L sodium heptanesulfonate buffer solution-methanol（60∶40） at a flow rate of 1.0 mL/min.The detection wavelength was 277 nm and the column temperature was 30 ℃.Results The mass concentration of chloramphenicol within the range of 0.01-1.0 g/L showed good linear relation with the peak area.The average recovery rate was 100.18 %,RSD =1.38%.Conclusion This method has high sensitivity,is easy to operate,accurate and reliable,which can be used for the content determination of chloramphenicol in chlorine zinc oil. |
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| Keywords: | chlorine zinc oil chloramphenicol HPLC |
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