| 高效液相色谱 - 蒸发光散射检测法测定脑络通胶囊中黄芪甲苷含量 |
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| 引用本文： | 岳莉. 高效液相色谱 - 蒸发光散射检测法测定脑络通胶囊中黄芪甲苷含量[J]. 中国药业, 2014, 0(8): 33-35 |
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| 作者姓名： | 岳莉 |
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| 作者单位： | 安徽省淮北市食品药品检验所,安徽淮北235000 |
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| 摘 要： | 目的采用高效液相色谱-蒸发光散射检测（ELSD—HPLC）法测定脑络通胶囊中黄芪甲苷的含量。方法采用HypersilODS2色谱柱（150mm×4．6mm，5μm），流动相为乙腈-水（30：70），流速为1．0mL／min，柱温为25℃；蒸发光散射检测器条件，漂移管温度50cc。结果黄芪甲苷质量浓度在0．0667～0．4668g／L（r=0．9999）范围内与峰面积呈良好线性关系，平均加样回收率为100．70％（n=9），RSD=1．28％。结论该方法操作简便、准确、稳定、灵敏度高，可作为该制剂的质量控制方法。
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| 关 键 词： | 高效液相色谱-蒸发光散射检测法 脑络通胶囊 黄芪甲苷 含量测定 |
Content Determination of Astraloside in Naoluotong Capsules by HPLC- ELSD |
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| Yue Li. Content Determination of Astraloside in Naoluotong Capsules by HPLC- ELSD[J]. China Pharmaceuticals, 2014, 0(8): 33-35 |
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| Authors: | Yue Li |
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| Affiliation: | Yue Li (Huaibei Municipal Institute for Food and Drug Control, Huaibei, Anhui, China 235000) |
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| Abstract: | Objective To determine the content of astraloside in Naoluotong Capsules by HPLC-evaporative light scattering detector （ELSD）. Methods The Hypersil ODS2 column （150 mm x4.6 mm,5 μm） was adopted,the mobile phase was acetonitrile-water（30 ： 70）,the flow rate was 1. 0 mL/min,the drift tube temperature was 105 ℃. The column temperature was 25 ℃. Results The linear range of astraloside was 0. 066 7-0. 466 8 mg/mL（ r=0. 999 9）,the average recovery rate was 100.70% （n=9）, RSD = 1. 28%. Conclusion This method is simple to operate ,accurate,stable ,and highly sensitive ,and can be used as the the quality control method of this preparation. |
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| Keywords: | ELSD - HPLC Naoluotong Capsules astraloside content determination |
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