高效液相色谱法同时测定连翘叶中连翘酯苷A和连翘苷含量

目的建立同时测定连翘叶中连翘酯苷A和连翘苷含量的高效液相色谱法。方法采用AgilentZorbaxSB—C18柱（150mm×4．6mm，5μm），以甲醇-0．1％磷酸溶液（32：68）为流动相，检测波长为227nm，流速为1．0mL／min，进样量为10txL，柱温为30℃。结果连翘酯苷A、连翘苷进样量分别在293．170～3664．624ng（r=0．999980）和333．760—4172．000ng（r=0．999991）范围内与相应峰面积皇良好线性关系，平均回收率分别为97．00％（RSD：1．88％）和99．97％（RSD=2．11％）。结论该法操作简便、结果准确、专属性强，可用于同时测定连翘叶中连翘酯苷A和连翘苷的含量。

Simultaneous Determination of Forsythoside A and Forsythin from Forsythia Suspense Leaves by HPLC

Objective To establish a high performance liquid chromatography（HPLC） method for the simultaneous determination of forsythoside A and forsythin contents from Forsythia suspense leaves. Methods The analysis was carried out by using the Agilent Zorabx SB-C15 column（150 mm×4.6 mm, 5 μm） and with methanol-0.1% phosphoric acid solution （32：68） as the mobile phase. The flow rate was 1.0 mL/min, the detection wavelength was 227 nm, the injection volume was 10 μL and the column temperature was 30℃. Results The linear ranges of forsythoside A and forsythin were 293. 170-3 664. 624 ng（ r =0. 999 980） and 333. 760- 4 172. 000 ng（ r = 0. 999 991 ） respectively. The average recovery rates were 97.00% （ RSD = 1.88% ） for forsythoside A and 99.97% （RSD = 2. 11% ） for forsythin. Conclusion This method is simple to operate and accurate in detection results with good strong speci- ficity, which can be used for the simultaneous determination of forsythoside A and forsythin in Forsythia suspense leaves.