他莫昔芬对人肝癌细胞增殖及ER、Smad4表达的影响

目的：研究他莫昔芬对人肝癌HepG2细胞增殖、雌激素受体（ER）、Smad4表达的影响。方法：根据他莫昔芬的不同浓度分为3组，即7.5、15、30μmol/L组，对照组不加他莫昔芬，每组设6个平行对照孔，每孔加入细胞浓度为1×105个/ml的细胞悬液0.1ml，培养24、48、72h。采用MTT法测定OD值，然后计算抑制率；免疫组化染色观察他莫昔芬对人肝癌HepG2细胞ER、Smad4表达的影响。结果：他莫昔芬抑制人肝癌细胞增殖，随浓度的增加及处理时间的延长，抑制率增加；他莫昔芬抑制肝癌细胞ER表达，促进Smad4的表达，具有时间－浓度依赖。结论：他莫昔芬可通过影响肝癌细胞ER、Smad4的表达来抑制肝癌细胞增殖。

Effect of tamoxifen on the proliferation and expression of ER and Smad4 in human hepatocellular carcinoma cells HepG2

Objective: To study the effect of tamoxifen on the proliferation and expression of the estrogen receptor(ER) and Smad4 in human hepatocellular carcinoma cells HepG2. Methods: Three experimental groups were subjected to 7.5μmol/L,　15μmol/L,　 and 30μmol/L tamoxifen. The control group was not subjected to tamoxifen. Each group contained six wells, and there were 0.1ml containing 1×105/ml cells in each well. After being observed for 24h, 48h, and 72h, the inhibiting rate of HepG2 cells was determined by MTT. The expression of ER and Smand4 in HepG2 cells was determined by immunohistochemistry. Results: Tamoxifen inhibited the proliferation of human hepatocellular carcinoma cells in a time and dose dependent manner. It also suppressed the ER expression but raised the Smad4 expression in a time and dose dependent manner. Conclusion: Tamoxifen can suppress human hepatocellular carcinoma cell proliferation by affecting the expression of ER and Smad4 in HepG2 cells.