生长因子诱导的血管内皮细胞增殖作用的研究

目的 研究生长因子对血管内皮细胞增殖活动的诱导和刺激作用。以进一步探讨生长因子在视网新生血管疾病中所起的作用。方法 采用血管内皮生长因子（VEGF）和碱性成纤维生长因子（bFGF）诱导离体培养的血管内皮细胞的增殖。研究VEGF及bFGF对血管内皮细胞DNA合成、细胞生长和细胞周期的调控，以及两种因子的协同效应。结果 VEGF及bFGF均可显著刺激血管内皮细胞的增生，其刺激效应与生长因子浓度呈剂量依赖关系。共同作用时，具有协同效应。两种因子均可显著增加^3H-TdR的掺入量，其刺激作用与浓度呈剂量依赖关系，共同作用时亦具有协同效应。VEGF和bFGF均可显著地产加S期细胞的比例。结论 VEGF及bFGF均可显著地刺激内皮细胞的增殖，促进细胞DNA的合成。两种因子联合应用时，具协同效应。提示：两种生长因子在视网膜新生血管的发生和发展中可能具有一定作用，且存在因子间的协同效应。

Growth factors induced vascular endothelial cell proliferation in vitro

Objective To demonstrate the effects of growth factors on the vascular endothelial proliferation. The possible roles and synergistic effect of growth factors on retinal neovascular disorders. Methods Vascular endothelial growth factor (VEGF) and basic fibroblastic growth factor (bFGF) were used to induce the proliferation of vascular endothelium in vitro. The regulatory effects of VEGF and bFGF on endothelium DNA synthesis, cell growth and cell phases were studied. The synergistic effect of two factors was also investigated. Results Both VEGF and bFGF significantly induced the endothelial proliferation in vitro in a dose dependent manner. In combination,a synergistic effect was produced. Both factors significantly increased the CPM of3H-TdR in endothelial cells. A synergistic effect was also observed in combination of two factors. Both growth factors induced increase of cell population in S phase. Conclusion VEGF and bFGF can induce vascular endothelial proliferation and promote DNA synthesis. It is suggested that both factors may possibly be involved in the etiology of retinal neovascularization.