焦炉工染色体畸变和血清p53蛋白水平的检测

目的：探讨职业性肺癌早期监测指标染色体畸变及血清ｐ５３蛋白的应用价值。方法：选择长期接触多环芳烃混合物（ＰＡＨｓ）的２３名焦炉工为研究对象，另选１２名非暴露者为对照，进行外周血淋巴细胞染色体畸变检查，对有畸变者采用双抗夹心酶联免疫吸附试验（Ｓａｎｄｗｉｃｈ－ＥＬＩＳＡ）测定血清ｐ５３蛋白水平。结果：焦炉工染色体异常检出率高于对照组（Ｐ＜０．０５），畸变类型为单体型，以裂隙为主；焦炉工血清ｐ５３水平均值高于对照，但差异无显著性，其中５例血清ｐ５３水平高于对照组ｘ＋２Ｓ，判为阳性；两指标间无相关关系。结论：染色体畸变及血清ｐ５３水平可从不同途径反映ＰＡＨｓ对机体遗传物质的损伤作用，两者的联合应用是提高肿瘤预报性的有效途径

The Detection of Chromosomal Aberration And Serum Level of p53 Protein in Coke Workers

Objective: To discuss the application value of chromosomal aberration (CA) and serum level of p53 protein to the early monitory of occupational lung cancer.Methods: 23 coke workers were chosen as the exposure group,who had long term exposure to PAHs,and the control group was composed of 12 non exposed people.They were primarily assayed by the peripheral lymphocyte chromosomal aberration.And 16 coke workers and 12 people for control were tested for serum level of p53 by Sandwich ELISA after reviewing the CA results.Results: The CA rates of coke workers were higher than that of control ( P <0.05).Chromosomal gap was the main type among all kinds of CA.The average value of coke workers’ serum p53 level was higher than that of control,but there was no significant difference.5 of the exposed group were determined as positive because their serum p53 levels were greater than 2SD above the control average.There was no linear correlation between CA and serum p53 level.Conclusion: CA and serum p53 level,by different paths,reflected the damage of genetic material caused by PAHs.Co application of both markers will be an effective path to improve prediction of cancer.