Guinea pig tectorial membrane profile in an in vitro cochlear preparation |
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| Authors: | S S Orman C D Geisler |
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| Institution: | 1. Department of Neurophysiology, University of Wisconsin-Madison, Madison, Wisconsin.;2. Department of Electrical and Computer Engineering, University of Wisconsin-Madison, Madison, Wisconsin.;1. Faculty of Engineering, Universiti Malaya, Malaysia;2. Faculty of Business and Economics, Universiti Malaya, Malaysia;1. Department of Nutrition, Faculty of Nursing and Nutrition, University of Nagasaki, Siebold, 1-1-1 Manabino, Nagayo-cho, Nishisonogi-gun, Nagasaki 851-2195, Japan;2. Graduate School of Human Health Science, University of Nagasaki, Siebold, 1-1-1 Manabino, Nagayo-cho, Nishisonogi-gun, Nagasaki 851-2195, Japan;3. Department of Applied Food Science, Higashiosaka Junior College, 3-1-1 Nishidutsumigakuen-cho, Higashiosaka city, Osaka 577-0044, Japan;4. Graduate School of Education, Hiroshima University, 1-1-1 Kagamiyama, Higashi-Hiroshima city, Hiroshima 739-8524, Japan;1. Department of Ophthalmology & Visual Science, Eye & ENT Hospital, Shanghai Medical College, Fudan University, Shanghai, 200031, China;2. State Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain Science, Institutes of Brain Science, Fudan University, Shanghai, 200032, China;3. NHC Key Laboratory of Myopia, Chinese Academy of Medical Sciences, And Shanghai Key Laboratory of Visual Impairment and Restoration (Fudan University), Shanghai, 200031, China;4. Department of Integrative Medicine and Neurobiology, State Key Lab of Medical Neurobiology, Institute of Integrative Medicine of Fudan University, Institute of Brain Science, School of Basic Medical Sciences, Fudan University, Shanghai, 200032, China |
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| Abstract: | The guinea pig cochlea was examined under high-magnification light microscopy in an in vitro preparation. After extraction of the otic capsule, the bulla was opened widely and a small hole made into the fourth turn of the scala vestibuli. The organ of Corti was visualized under artificial endolymph at 600 X magnification. Added 1-micron titanium dioxide particles settled on the upper surface of the transparent tectorial membrane. Particle positions showed that much of this upper surface lay in a flat sheet that extended centrifugally almost to the Hensen's cells, giving the impression it was attached there. The sheet extended at least to the level of the inner hair cells, where a tectorial membrane thickness of about 40 micron was reached. Titanium dioxide particles were seen regularly in immediate proximity to the hair cell cilia, indicating that scala media is continuous with the subtectorial space. Upon mechanical manipulation, Hensen's cells proved to be extremely cohesive and elastic. It is suggested that hair cell stereocilia provide major mechanical connections for the tectorial membrane. |
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