Neuropeptide Y increases the inhibitory effects of clonidine on potassium evoked3H-noradrenaline but not3H-5-hydroxytryptamine release from synaptosomes of the hypothalamus and the frontoparietal cortex of the male Sprague-Dawley rat |
| |
Authors: | M. Martire K. Fuxe G. Pistritto P. Preziosi L. F. Agnati |
| |
Affiliation: | (1) Department of Pharmacology, Catholic University, Rome, Italy;(2) Present address: Department of Histology and Neurobiology, Karolinska Institute, Stockholm, Sweden;(3) Department of Human Physiology, University of Modena, Modena, Italy |
| |
Abstract: | Summary The release of3H-noradrenaline (3H-NA) and of3H-5-hydroxytryptamine (3H-5-HT) evoked by high-K+ (15 mM) was studied in synaptosomes isolated from the hypothalamus and the frontoparietal cortex of the male Sprague-Dawley rat using a superfusion apparatus. Based on concentration-response curves obtained by analyzing the full-time course of the inhibitory effects of clonidine on3H-NA and on3H-5-HT release neuropeptide Y (NPY) (1 nM) was shown to significantly increase the ability of clonidine to inhibit3H-NA release in synaptosomes isolated from the hypothalamus and from the frontoparietal cortex. NPY (1 nM) alone had no effect on K+-evoked3H-NA release from these regions. In contrast, NPY (1 nM) did not modulate the inhibitory effects of clonidine on3H-5-HT release in the above mentioned regions. These results indicate that NPY can increase the sensitivity of the 2-autoreceptors belonging to hypothalamic NA and/or to adrenaline nerve terminals and to cortical NA nerve terminals, while the 2-heteroreceptors inhibiting3H-HT release in the same brain regions appear not to be regulated by high affinity NPY receptors. Thus, 2-autoreceptors and 2-heteroreceptors appear to be differentially controlled by high affinity NPY receptors at least with regard to regulation of3H-NA and3H-5-HT release, respectively. |
| |
Keywords: | Neuropeptide Y clonidine /content/x1816w5x88888j5k/xxlarge945.gif" alt=" agr" align=" BASELINE" BORDER=" 0" >2-autoreceptors /content/x1816w5x88888j5k/xxlarge945.gif" alt=" agr" align=" BASELINE" BORDER=" 0" >2-heteroreceptors noradrenaline release 5-hydroxytryptamine release synaptosomes hypothalamus cortex cerebri |
本文献已被 SpringerLink 等数据库收录! |