高血压大鼠脑缺血再灌注损伤的观察

目的：观察大鼠脑缺血再灌注损伤。方法：选ＳＤ 大鼠，制备肾血管性高血压大鼠（ＲＨＲ），以线栓法复制大脑中动脉梗阻（ＭＣＡＯ） ３ ｈ 后再灌注２１ ｈ（Ａ 组）；ＭＣＡＯ６ ｈ 后再灌注１８ ｈ（Ｂ组）；ＭＣＡＯ２４ ｈ（Ｃ组）。于ＭＣＡＯ 后６ ｈ 及２４ ｈ 进行神经功能障碍评分，ＴＴＣ染色后以图像分析技术测脑梗塞体积。结果：Ａ 组大部分动物无神经功能障碍及梗死灶出现；Ｂ、Ｃ两组均全部出现不同程度神经功能障碍及梗死灶，且Ｂ组较Ｃ组更严重，具有显著性差异（Ｐ＜ ０．０１）。结论：高血压大鼠线栓法脑缺血６ ｈ 后再灌注１８ ｈ 可引起显著的再灌注损伤

REPERFUSION INJURY IN CEREBRAL ISCHEMIA RATS WITH HYPERTENSION

Objective: To study the reperfusion injury in rats with cerebral ischemia. Method: Renovascular hypertension was induced in Sprague Dewley(SD)rats for the study. Middle cerebral artery occlusion (MCAO) was done for 3 hours with reperfusion for 21 hours in Group A, MCAO for 6 hours with reperfusion for 18 hours in Group B and MCAO for 24 hours with no reperfusion in Group C respectively. Neurological deficit evaluation was then carried out at 6 hours and 24 hours after MCAO and cerebral infarction volume was examined with computer imaging techniques after TTC staining. Results: Most rats in Group A had no neurological deficit and/or cerebral infarction while Groups B and C had these problems to various degrees. Group B showed even more serious neurological deficit and greater cerebral infarction volume than Group C(P<0.01). Conclusion: Significant reperfusion injury may develop in rats with renovascular hypertension at 6 hours after cerebral ischemia with reperfusion for 18 hours afteh MCAO.