| 半夏工厂化育苗技术体系研究初探 |
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| 引用本文： | 张晓伟,王小峰,周昌华,张兴翠.半夏工厂化育苗技术体系研究初探[J].医学教育探索,2007(3):432-435. |
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| 作者姓名： | 张晓伟 王小峰 周昌华 张兴翠 |
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| 作者单位： | 西南大学农学与生命科学学院 重庆400716(张晓伟，王小峰，张兴翠)，西南大学科技处 重庆400716(周昌华) |
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| 摘 要： | 目的建立一套半夏工厂化育苗体系,实现半夏的快速繁殖,解决半夏生产因用种而造成的退化问题。方法探讨了外植体诱导、植物生长调节物质质量浓度、光照条件及继代周期对丛生芽增殖的影响。结果外植体的诱导以MS 6-BA(4~5)mg/L NAA0.2mg/L培养基为宜。组织培养中生长调节物质质量浓度为NAA0.2mg/L 6-BA2mg/L、转接时组织块带两个或两个以上芽时,半夏生长状况最好。结论采用工厂化育苗技术可进行半夏的快速繁殖,为保护和持续利用这一重要药材资源提供有效途径。
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| 关 键 词： | 半夏 组织培养 工厂化育苗体系 |
Preliminary research on industrialized breeding technology system of Pinellia ternata |
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| ZHANG Xiao-wei,WANG Xiao-feng,ZHOU Chang-hu,ZHANG Xing-cui.Preliminary research on industrialized breeding technology system of Pinellia ternata[J].Researches in Medical Education,2007(3):432-435. |
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| Authors: | ZHANG Xiao-wei WANG Xiao-feng ZHOU Chang-hu ZHANG Xing-cui |
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| Institution: | 1. College of Agronomy and Life Science, Southwest University, Chongqing 400716, China; 2. Department of Science and Technology, Southwest University, Chongqing 400716, China |
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| Abstract: | Objective Industrialized breeding technology system will be established to achieve the rapid multiplication of Pinellia ternata and to settle the problem of degeneration caused by seeds. MethodsExplant induction and effects of plant growth regulator dosage, illumination, and subculture cycle on fasciculated shoot propagation were studied. ResultsIt is good for the explant induction on medium MS 6-BA (4-5) mg/L NAA 0.2 mg/L. P.ternata grows well under the circumstance of NAA 0.2 mg/L 6-BA 2 mg/L, double or more shoots. ConclusionIndustrialized breeding technology system of P.ternata can make its propagation rapid and support an effective way for resources preserving and sustainable utilization. |
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| Keywords: | Pinellia ternata (Thunb ) Breit tissue culture industrialized breeding system |
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