| 苦参碱抑制人大肠癌HT29细胞增殖及诱导凋亡作用与机制 |
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| 引用本文： | 黄建,陈康杰,张卧,朱永良.苦参碱抑制人大肠癌HT29细胞增殖及诱导凋亡作用与机制[J].医学教育探索,2007(8):1210-1214. |
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| 作者姓名： | 黄建 陈康杰 张卧 朱永良 |
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| 作者单位： | 浙江大学医学院附属第二医院肿瘤科 浙江杭州310009(黄建，张卧)，温州医学院附属第二医院普外科 浙江温州325000(陈康杰)，浙江大学医学院附属第二医院消化科 浙江杭州310009(朱永良) |
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| 基金项目： | 浙江省自然科学基金;浙江省中医药管理局科研项目 |
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| 摘 要： | 目的探讨苦参碱对人大肠癌HT29细胞增殖抑制和凋亡诱导作用及其可能机制。方法分别采用MTT法检测细胞增殖活性、流式细胞仪检测细胞周期及凋亡、透射电镜观察细胞结构变化、基因芯片检测细胞基因表达改变。结果0.0625～0.5mg/mL苦参碱处理48h后,细胞增殖明显受抑制;但1mg/mL时增殖抑制率降低而凋亡诱导作用明显增强;苦参碱对细胞G2/M和G1/S期均有阻滞作用;电镜下可见凋亡的形态学变化;基因芯片检测发现苦参碱影响细胞增殖、周期和凋亡相关基因的表达。结论苦参碱通过影响HT29细胞一些与增殖、周期和凋亡相关基因的表达发挥增殖抑制和凋亡诱导作用,且与苦参碱质量浓度相关。
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| 关 键 词： | 苦参碱 增殖 凋亡 基因芯片 |
| 修稿时间： | 1/5/2007 12:00:00 AM |
Effect of matrine on inhibiting proliferation and inducing apoptosis of human intestinum crassum carcinoma HT29 cells |
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| HUANG Jian,CHEN Kang-jie,ZHANG Wo,ZHU Yong-liang.Effect of matrine on inhibiting proliferation and inducing apoptosis of human intestinum crassum carcinoma HT29 cells[J].Researches in Medical Education,2007(8):1210-1214. |
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| Authors: | HUANG Jian CHEN Kang-jie ZHANG Wo ZHU Yong-liang |
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| Institution: | 1. Department of Oncology, Second Affiliated Hospital of Medical School, Zhejiang University, Hangzhou 310009, China; 2. Department of Surgery, Second Affiliated Hospital of Wenzhou Medical School, Wenzhou 325000, China; 3. Department of Gastroentology, Second Affiliated Hospital of Medical School, Zhejiang University, Hangzhou 310009, China |
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| Abstract: | Objective To explore the anti-proliferation and apoptosis-induced effects of matrine on HT29 cell and their possible mechanism.Methods HT29 Cell proliferative activity was measured by MTT assay;Cell cycle and apoptosis were analyzed by Flow Cytometry;Alteration of cellular skeleton was observed by transmission electron microscopy(TEM);Global gene expression profiles were scanned by gene chip.Results After exposure to matrine at concentrations from 0.062 5 to 0.5 mg/mL for 48 h,cellular proliferation was inhibited with increasing the concentration,but this inhibitory effect attenuated at 1 mg/mL and the apoptosis was up-regulated significantly.G2/M and G1/S phases of cell cycle were,to some extent,arrested.Under TEM,the morphological changes could be found.Gene chip showed that matrine could alter a large number of genes,which related to the cell proliferation,cell cycle,and apoptosis.Conclusion The anti-proliferation and apoptosis-induced effects of matrine on HT29 cells are concentration-dependent through changing of many genes involved in proliferation,cell cycle,and apoptosis. |
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| Keywords: | matrine proliferation apoptosis gene chip |
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