| 黄花蒿种质资源的RAPD分析 |
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| 引用本文： | 郑丽屏,王剑文,谭仁祥.黄花蒿种质资源的RAPD分析[J].医学教育探索,2007(4):602-605. |
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| 作者姓名： | 郑丽屏 王剑文 谭仁祥 |
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| 作者单位： | 苏州大学城市科学学院园艺系 江苏苏州215123(郑丽屏)，苏州大学药学院 江苏苏州215123(王剑文)，南京大学功能生物分子研究所 江苏南京210093(谭仁祥) |
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| 基金项目： | 国家自然科学基金;江苏省高校自然科学基金;苏州大学校科研和教改项目 |
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| 摘 要： | 目的研究青蒿素高产的黄花蒿植株的遗传多样性特征。方法结合青蒿素量的测定,应用RAPD技术对10个产地黄花蒿进行遗传多样性分析。结果发现RAPD多态位点为53.6%,证明在黄花蒿野生群体中存在较丰富的遗传多样性。UPGMA分析表明:在黄花蒿中可能至少存在具有遗传分化的4个分支,黄花蒿遗传分化与青蒿素量的变化及地理分布有一定的相关性。结论黄花蒿具有明显的遗传分化,这些遗传分化是黄花蒿种质资源筛选的关键,是青蒿素“高量育种”和生物技术开发的基础。
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| 关 键 词： | 黄花蒿 青蒿素 |
RAPD Analysis of germplasm resource in Artemisia annua |
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| ZHENG Li-ping,WANG Jian-wen,TAN Ren-xiang.RAPD Analysis of germplasm resource in Artemisia annua[J].Researches in Medical Education,2007(4):602-605. |
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| Authors: | ZHENG Li-ping WANG Jian-wen TAN Ren-xiang |
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| Institution: | 1. Department of Horticulture, School of Urbanology, Soochow University, Suzhou 215123, China; 2. School of Pharmaceutical Sciences, Soochow University, Suzhou 215123, China; 3. Institute of Functional Biomolecules, Nanjing University, Nanjing 210093, China |
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| Abstract: | Objective To analyze the genetic diversity of higher artemisinin-yielding Artemisia annua. Methods With the chemical investigation of artemisinin contents, RAPD analyses of selected plants from ten various habitats in China were carried out using arbitrary primers. Results The RAPD data clearly indicated the genetic diversity level in A. annua was relatively high with 53.6% polymorphic sites. UPGMA Analyses of RAPD and phytochemical trait data showed that the wide phytochemical diversity was included within the genetic diversity being present in at least four geographical regions. ConclusionArtemisinin has obvious inherited differentiation that further support the prospects for selection and breeding of superior artemisinin containing lines. |
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| Keywords: | RAPD |
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