吡喹酮对日本血吸虫雄虫的Ca2+,Mg2+,K+与Na+的含量及45Ca2+在虫体内分布的影响

日本血吸虫雄虫在4℃或37℃的HBS及无⁴⁵Ca²⁺的HBS中经吡喹酮1或30μg/ml作用0.5～2h后,未见虫的Ca²⁺,Mg²⁺含量有明显变化,但除4℃的HBS组外,余2组虫的K⁺含量明显减少,而虫的Na⁺含量的增加则不明显。在含30 mM Mg²⁺的HBS中,雄虫经吡喹酮作用1h后,虫的Mg²⁺含量明显增加。在37℃的HBS中,血吸虫雄虫经吡喹酮1μg/ml作用5～60min后,虫的皮层胞质中的⁴⁵²⁺含量的百分比较各相应对照组的明显减少,而虫体肌肉的则相反。在4℃的HBS或无⁴⁵Ca²⁺的HBS中,吡喹酮对⁴⁵²⁺在虫体内的分布无明显影响。

EFFECT OF PRAZIQUANTEL ON Ca2+, Mg2+, K+ AND Na+ CONTENT AND DISTRIBUTION OF 452+ IN MALE SCHISTOSOMA JAPONICUM

When male Schistosoma japonicum maintained in HBS at 4 or 37℃ and HBS without calcium were exposed to praziquantel 1 or 30 μg/ml for 0.5～2h, no apparent change in Ca²⁺ content of the worms was observed. With the exception of the group HBS at 4℃, a significant decrease in K⁺ content of the worms was detected in another 2 groups. On the other hand, Na⁺ content of the worms exhibited somewhat increase after treatment with praziquantel but not significantly. Incubation of male schistosomes in HBS with 30 mM Mg²⁺ resulted in an increase in Mg²⁺ content of the worms caused by praziquantel. Similar changes, compared to HBS group at 37℃, in the 4 element values were detected in male worms perfu sed out from infected mice treated orally with praziquantel 100～300 mg/kg. When male schistosomes maintained in HBS at 37℃ were exposed to prazquantel 1 μg/ml for 5～60 min the percentage of radioactivity distributed in tegumental cytoplasma decreased significantly while that of the worm musculature increased significantly. When male worms were incubated in HBS at 4℃ or HBS without calcium, no apparent change in distribution of radioactivity caused by praziquantel was detected.