基于网络药理学研究肝复方治疗肝癌的分子机制

目的:基于网络药理学方法,探究肝复方有效活性物质治疗肝癌的相关作用靶点及潜在分子机制.方法:通过检索中药系统药理学数据库与分析平台(TCMSP)及国内外文献筛选出肝复方药味中的化合物以及相关靶点蛋白.通过Gen-eCards数据库获取肝癌相关疾病靶点.运用Cytoscape 3.2.1软件构建药物-有效成分-靶点网...

Molecular mechanism of Ganfufang in treatment of liver cancer based on network pharmacology

Objective:To investigate the targets and potential molecular mechanism of the active components of Ganfufang in the treatment of liver cancer based on network pharmacology.Methods:Traditional Chinese Medicine Systems Pharmacology and related articles in China and globally were used to screen out the compounds of the herbal medicines in Ganfufang and related target proteins.The GeneCards database was used to obtain the targets of liver cancer.Cytoscape 3.2.1 was used to construct adrug-effective constituent-targetnetwork and a protein-protein interaction network,and a network topology analysis was performed to screen out core targets.Finally,Database for Annotation,Visualization and Integrated Discovery was used to perform gene ontology and KEGG pathway enrichment analyses.Results:A total of 104 effective constituents were screened out based on the criteria of oral bioavailability≥30%and drug-likeness≥0.18,with 1135 corresponding targets,and there were 16490 targets associated with liver cancer.Stigmasterol,ginsenoside,andβ-sitosterol were the core constituents,and interleukin-6(IL-6),epidermal growth factor receptor(EGFR),and CASP3 were key targets.Conclusion:Ganfufang has the features of multiple constituents,targets,and pathways in the treatment of liver cancer,possibly by regulating the core targets such as IL-6,vascular endothelial growth factor A,EGFR,and CASP3,with the involvement of the VEGF,PI3 K-Akt,and p53 signaling pathways.This study predicts the molecular mechanism of Ganfufang in the treatment of liver cancer based on network pharmacology and provides a theoretical basis for clinical application.