Diversity of Borrelia genospecies in Ixodes ricinus ticks in a Lyme borreliosis endemic area in Switzerland identified by using new probes for reverse line blotting |
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| Authors: | Lise Gern Véronique Douet Zully López Olivier Rais Francisca Morán Cadenas |
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| Affiliation: | 1. Bavarian Health and Food Safety Authority, Branch Oberschleißheim, Veterinärstr. 2, 85764 Oberschleißheim, Germany;2. National Reference Centre for Borrelia, Veterinärstr. 2, 85764 Oberschleißheim, Germany;3. The Robinson Research Institute, School of Paediatrics and Reproductive Health, The University of Adelaide, Adelaide 5005, SA, Australia;4. Comparative Tropical Medicine and Parasitology, LMU Munich, Munich, Germany;5. Dr. Gernot Walder GmbH, Unterwalden 30, 9931 Ausservillgraten, Austria;6. Department of Hygiene, Medical Microbiology and Social Medicine, Innsbruck Medical University, Fritz Pregl Str. 3, 6020 Innsbruck, Austria;1. Department of Environmental Science, Policy and Management, University of California, Berkeley, CA 94720, USA;2. Los Angeles County West Vector and Vector-Borne Disease Control District, Culver City, CA, 90230, USA;1. Federal State Public Science Institution, Scientific Centre of Family Health and Human Reproduction Problems, Irkutsk, Russia;2. Department of Veterinary Medicine, Joint Faculty of Veterinary Medicine, Yamaguchi University, Yamaguchi, Japan;3. Department of Bacteriology, National Institute of Infectious Diseases, Shinjuku-ku, Tokyo, Japan;4. United Graduate School of Agricultural Science & Veterinary Science, Gifu University, Gifu, Japan;5. School of Food and Nutritional Sciences, University of Shizuoka, Shizuoka, Japan;6. Faculty of Pharmaceutical Sciences, Chiba Institute of Science (CIS), Choshi, Japan;1. Institute of Medical Microbiology and Infection Control, University Hospital of Frankfurt, Paul-Ehrlich-Str. 40, D-60596 Frankfurt, Germany;2. German National Reference Centre for Borrelia, Oberschleissheim, Germany;1. Department of Preventive Medicine, Bundeswehr Medical Academy Munich, Neuherbergstraße 11, 80937 Munich, Germany;2. Department of Tropical Medicine at the Bernhard Nocht Institute, Bundeswehr Hospital Hamburg, Bernhard Nocht Str. 74, 20359 Hamburg, Germany;3. Institute for Microbiology, Virology and Hygiene, University Medicine Rostock, Schillingallee 70, 18057 Rostock, Germany;4. Biological Faculty, University of Hamburg, Martin-Luther-King-Platz 3, 20146 Hamburg, Germany;5. National Reference Center for Borrelia, Bayerisches Landesamt für Gesundheit und Lebensmittelsicherheit (LGL), Branch Oberschleißheim, Veterinärstraße 2, 85764 Oberschleißheim, Germany;6. Department of Microbiology and Parasitology, University of Antananarivo, BP 566, Antananarivo 101, Madagascar;7. International Vaccine Institute, 1 Gwanak-ro, Nakseongdae-dong, Gwanak-gu, Seoul, South Korea;8. Infectious Disease Epidemiology, Bernhard Nocht Institute for Tropical Medicine, Bernhard Nocht Str. 74, 20359 Hamburg, Germany;9. University Medical Center, University Hospital Hamburg-Eppendorf, Martinistraße 52, 20246 Hamburg, Germany |
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| Abstract: | In Europe, 7 Borrelia species belonging to the Borrelia burgdorferi sensu lato complex have been reported in Ixodes ricinus ticks. In addition, another Borrelia, related to the relapsing fever spirochaetes, has also been described. In the present study, we designed probes for reverse line blotting allowing detection and identification of all these Borrelia species after amplification of the variable spacer region between the 23S and 5S ribosomal genes. These new probes allowed us investigate the diversity of Borrelia in 915 I. ricinus collected on the south-facing slope of Chaumont (Switzerland). Among the 159 infected ticks, 7 Borrelia species were identified, and B. spielmanii and relapsing fever-like (RFL) spirochaetes were identified in this area for the first time. B. valaisiana and B. spielmanii were significantly less present in male than in female or nymphal ticks. Mixed infection with RFL spirochaetes and Lyme borreliosis spirochaetes were detected in 4 ticks. In addition, the set of probes could identify the recently described species, B. bavariensis. |
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