细胞因子微球检测技术测定异种脱细胞真皮基质口腔修复膜的细胞因子水平

目的:采用细胞因子微球检测技术测定异种脱细胞真皮基质口腔修复膜免疫评价中细胞因子的含量。方法:将异种脱细胞真皮基质口腔修复膜为试验样品,按照高、中、低剂量组植入至Balb/C小鼠皮下28 d。28 d后用细胞因子微球检测技术测定IL-1α、IL-1β、IL-6、IL-10、IL-17A、IL-23、肿瘤坏死因子α(TNF-α)、γ干扰素(IFN-γ)、β干扰素(IFN-β)、单核细胞趋化蛋白1(MCP-1)、粒细胞-巨噬细胞集落刺激因子(GM-CSF),将试验组与对照组的数据进行统计学分析。结果:阳性对照组中IL-1α、IL-1β、IL-6、IL-17A、TNF-α、IFN-γ、MCP-1、GM-CSF含量显著性高于假手术组(P<0.01),而IL-10、IL-23、IFN-β含量未见明显差异;被测样品植入试验组中,除MCP-1和TNF-α高剂量组显著性高于假手术组(分别为P<0.01和P<0.05),其他组的IL-1α、IL-1β、IL-6、IL-10、IL-17A、IL-23、TNF-α、IFN-γ、IFN-β、MCP-1、GM-CSF含量没有明显变化(P>0.05)。结论:采用细胞因子微球检测技术测定细胞因子水平,异种脱细胞真皮基质口腔修复膜对机体细胞因子无明显影响。

Determination of cytokine levels in dental restoration membrane of acellular dermal matrix by cytometric bead array

Objective:To determine the cytokine levels in immunity evaluation of dental restoration membrane of xenogeneic acellular dermal matrix using cytometric beads array(CBA).Methods:The dental restoration membrane of xenogeneic acellular dermal matrix was selected as a test sample and implanted into Balb/C mice subcutaneously to 28 days according to the high,medium and low dose groups.After 28 days,IL-1α,IL-1β,IL-6,IL-10,IL-17 A,IL-23,TNF-α,IFN-γ,IFN-β,MCP-1 and GM-CSF were measured by CBA.The data of the test group and the control group were analyzed statistically.Results:Compared with the negative control group,there was no significant difference in the IL-10、IL-23、IFN-β content in the positive control group,while the IL-1α,IL-1β,IL-6,IL-17 A,TNF-α,IFN-γ,MCP-1 and GM-CSF contents were significantly higher than the sham control group(P<0.01).In the sample groups,except for the high dose group of MCP-1 and TNF-α,which was significantly higher than the sham operation group(P<0.01 and P<0.05,respectively).The contents of IL-6,IL-10,IL-17 A,IL-23,TNF-α,IFN-γ,IFN-β,MCP-1,GM-CSF were not changed significantly(P>0.05).Conclusion:The dental restoration membrane of xenogeneic acellular dermal matrix have no significant effect on cytokines,as show at the levels of cytokines measured using CBA method.