雪松松针HPLC特征图谱及6个成分的含量测定

目的:利用高效液相色谱法建立雪松松针的特征图谱,同时测定其原儿茶酸、原儿茶醛、二氢杨梅素、咖啡酸、阿魏酸、芦丁6个成分的含量.方法:采用CAPCELL PAK-C1s色谱柱(250 mm×4.6 mm,5μm),以甲醇(A)-0.1％磷酸水(B)为流动相,梯度洗脱,流速为l mL· min-1,检测波长300 nm,柱...

HPLC characteristic chromatogram and assay of six components for pine needles of Cedrus deodara

Objective:To establish an HPLC characteristic chromatogram of pine needles of cedrus deodara and to develop simultaneous determination method of protocatechuic acid,protocatechuic aldehyde,dihydromyricetin,caffeic acid,ferulic acid and rutin in the sample.Methods:HPLC analysis was performed on a CAPCELL PAK-C18 column(250 mm×4.6 mm,5μm).The mobile phase consisted of methanol(A)and 0.1% phosphoric acid aqueous solution(B)with gradient elution.The flow rate was 1 mL·min^-1,the detection wavelength was 300 nm and the column temperature was 30℃.TCM chromatographic fingerprint similarity evaluation software(2012.130723 version)was used for similarity analysis in 14 batches of pine needles of Cedrus deodara,and the contents of six components in 14 batches of samples were determined at the same time.Results:The similarities between the characteristic chromatogram and chromatograms of 14 batches of pine needles in Cedrus deodara were between 0.932 and 0.992.There were altogether 22 common peaks,and six of them were identified.The contents of protocatechuic acid,protocatechuic aldehyde,dihydromyricetin,caffeic acid,ferulic acid and rutin in 14 batches of samples were in the range of 34.312^-1216.750μg·g^-1,5.246^-12.533μg·g^-1,43.912^-132.047μg·g^-1,50.791^-145.021μg·g^-1,13.543-70.379μg·g^-1 and 82.763-243.758μg·g^-1,respectively.Conclusion:The combination of the HPLC characteristic chromatogram and content determination of six components can reflect inherent quality of pine needles of Cedrus deodara.The proposed method is simple,feasible,rapid and accurate,providing reference for quality evaluation of the drug.